Enzyme Engineering: Volume 3

Innovation in Enzyme Engineering.- Session I. New Methods of Stabilizing and Immobilizing Enzymes and Whole Cells.- The Immobilization of a Stable Esterase by Entrapment, Covalent Binding and Adsorption.- Liquid-Membrane Hollow Fiber Enzyme Reactors.- Synthesis of Organic Acids and Modification of Steroids by Immobilized Whole Microbial Cells.- Immobilization of Enzymes by Reductive Alkylation.- Development of Immobilized Urease for the Owens-Illinois BUN Analyzer.- Immobilization of ?-Tyrosinase Cells with Collagen.- Stepwise Thermophotochemical Crosslinking for Enzyme Stabilization and Immobilization.- Session II. Enzyme Engineering.- Mass Transfer Problems with Immobilized Oxidases.- Mass Transfer and Reaction with Microcapsules Containing Enzyme and Adsorbent.- Enzymatic Regeneration of ATP from AMP and ADP: Kinetic Studies with the Coupled Enzyme System.- Cofactor Regeneration in Artificial Enzyme Membranes: Potentialities for Analytical and Reactor Applications.- Optimal Operations of a Batch Enzyme Reactor: Isomerization of D-Glucose to D-Fructose.- Physicochemical Aspects of Immobilized Enzyme-Matrix Interactions.- Coupled and Multienzyme-Nylon Tube Reactors.- Short Reports: Kinetics and Stability of Immobilized Glucose Oxidase and Catalase.- Kinetics of D-Glucose to D-Fructose Isomerization.- Efficiency of Two Immobilized Enzymes Acting in Sequence.- Photocontrol of Enzyme-Collagen Membrane Activity.- Enzyme Immobilization in Collagen Films Adhered to Semi-Rigid Supports: The Enzymatic Sponge Reactor.- Hollow Fiber Enzymatic Reactors: An Engineering Approach.- Session III, Enzyme Engineering and Enzyme Purification.- Genetic Engineering with Nitrogen Fixation.- The State of Enzyme Isolation Technology.- Unit Operations of Enzyme Purification.- Scale-Up of Enzyme Purification.- Carbon Dioxide as a Reagent for Proteins.- Application of Computers to Enzyme Recovery.- Studies on Immobilized Trypsin in High Concentrations of Organic Solvents.- A Tapered Fluidized Bed as a Bioreactor.- Short Reports: Induction of Phenol Hydroxylase in Yeast.- Session IV. Affinity Chromatography and Enzyme Cofactor Immobilization, Application and Regeneration.- Analysis of Ligand-Macromolecule Interactions by Quantitative Affinity Chromatography.- Affinity Chromatography: Interferences and their Prevention.- Immobilized Cofactors and Cofactor Fragments in General Ligand Affinity Chromatography and as Active Cofactors.- Purification of Dehydrogenases and Kinases by Affinity Chromatography.- Factors Concerning the Effectiveness of Affinity Chromatography Using Immobilized Cofactors.- Hydrophobic Chromatography and Its Potential Use in Biotechnology.- Purification of Proteinases by Affinity Chromatography Techniques.- Covalent Affinity Chromatography of Acetylcholinesterase.- Salt-Stable Hydrophobic Versus Salt-Reversible Electrostatic Effects in Adsorptive Protein Binding.- Strategy for Pure Hydrophobic Chromatography.- Short Reports: High Turnover NAD Regeneration in the Coupled Dehydrogenase Conversion of Sorbitol to Fructose.- Microencapsulated Multi-Enzyme Systems as Vehicles for the Cyclic Regeneration of Free and Immobilized Coenzymes.- Session V. Analytical and Biomedical Applications of Immobilized Enzymes.- Analytical and Biomedical Applications of Immobilized Enzymes: A Review.- Refixation of Solubilized and Purified Microsomal Enzymes: Towards an Extracorporal Detoxification in Liver Failure.- Immobilized Enzymes in Tubes and Hollow Fibers for Clinical Applications.- The Cholesterol Electrode: Use of the Polarographic Oxidase Anode with Multiple Enzymes.- Medical Applications of Affinity Chromatography.- Studies on the Thermal Enzyme Probe.- Prospects for an Assay Technique Based on Volatile Enzyme Products.- Short Reports: Enzymatic Catalysis of a Three Step Steroid Conversion.- Enzyme-Thermistor Assays of Cholesterol, Oxalic Acid, Glucose and Lactose in Standard Solutions and Biological Samples.- Session VI. Industrial Applica