Cell Culture Engineering VI

Cell therapy in kidney failure; H.D. Hunes, et al. Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE); T.L. Aldrich, et al. Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination; A. Hawerkamp, et al. Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line; B. Rasmussen, et al. Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them; S.J. Wessmann, R.L. Levings. An overview of viral and viral-like agents in cell culture systems; J.C. Petricciani. New adenovirus vectors for protein production and gene transfer; B. Massie, et al. Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue; F. Franek, et al. Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth-associated production of a foreign protein, beta-galactosidase; F.W.F. Lee, et al. A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy; G. Schoofs, et al. Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3; N-A. Sunstrom, et al. Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions; J. Varani, et al. Regulated multicistronic expression technology for mammalian metabolic engineering; M. Fussenegger, et al. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions; A. de Léon, et al. Historical reflections on cell culture engineering; A.S.Lubiniecki. Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation; J.H. Parham, et al. Population balance model of in vivo neutrophil formation following bone marrow rescue therapy; L.K. Nielsen, et al. Mammalian cell retention devides for stirred perfusion bioreactors; S.M. Woodside, et al. Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells; A. Perani, et al. Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions; S. Mercille, B. Massie. Effects of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures; E-J. Schlaeger, K. Lundstrom. Effects of CO2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production; V.M. deZengotita. pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons; M. Fussenegger, et al.