Proteins

The Lowry Method for Protein Quantitation.- Determination of Protein Molecular Weights by Gel Permeation High Pressure Liquid Chromatography.- Immunoaffinity Purification of Protein Antigens.- Electrophoretic and Chromatographic Separation of Peptides on Paper.- Peptide Mapping by Reverse-Phase High Pressure Liquid Chromatography.- SDS Polyacrylamide Gel Electrophoresis of Proteins.- Gradient SDS Polyacrylamide Gel Electrophoresis.- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins.- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis.- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins.- Starch Gel Electrophoresis of Proteins.- Isoelectric Focusing in Ultrathin Polyacrylamide Gels.- High-Sensitivity Silver Staining of Proteins Following Polyacrylamide Gel Electrophoresis.- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive).- Computer Analysis of Gel Scans.- Drying Gels.- Fluorography of Polyacrylamide Gels Containing Tritium.- Recovery of Proteins from Dried Polyacrylamide Gels after Fluorography.- The Electrophoretic Elution of Proteins from Polyacrylamide Gels.- Transfer Techniques in Protein Blotting.- Peptide Mapping by Thin-Layer Chromatography and High Voltage Electrophoresis.- In Situ Peptide Mapping of Proteins Following Polyacrylamide Gel Electrophoresis.- The Dansyl Method for Identifying N-Terminal Amino Acids.- The Dansyl-Edman Method for Peptide Sequencing.- Microsequencing of Peptides and Proteins with 4-N,N-Dimethylaminoazobenzene-4?-isothiocyanate.- Manual Edman Degradation of Proteins and Peptides.- Carboxyl-Terminal Sequence Determination of Proteins and Peptides with Carboxypeptidase Y.- Immunization and Fusion Protocols for Hybridoma Production.- Immunofluorescence and Immunoperoxidase Screening of Hybridomas.- Solid-Phase Screening of Monoclonal Antibodies.- Subclass Analysis and Purification of Monoclonal Antibodies.- The Production of Antisera.- Immunodiffusion in Gels.- Crossed Immunoelectrophoresis.- Rocket Immunoelectrophoresis.- Radioiodination of Proteins.- Radioimmunoassay.- Enzyme-Linked Immunosorbant Assay (ELISA).