Retinoid Protocols

Part I. Handling and Analysis of Retinoids
Properties of Retinoids:Structure, Handling, and Preparation
Arun B. Barua and Harold C. Furr
Quantitative Analyses of Naturally Occurring Retinoids
Joseph L. Napoli and Ronald L. Horst
Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines
Michael Wagner
Part II. Retinoid-Binding Proteins
Immunohistochemistry for CRBPs and CRABPs
Ulf Eriksson and Anne-Lee Gustafsson
Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid
Sally D. Lyn
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins
Andrew D. Loughney and Christopher P. F. Redfern
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
John W. Crabb, Yang Chen, Steve Goldflam, Karen West, and James Kapron
Expression and Purification of CRABPs from E. coli
Joseph L. Napoli, Zuzana Sperkova, Marek Sperka, and Paul D. Fiorella
Purification and Fluorescent Titration of Cellular Retinol-Binding Protein
Giorgio Malpeli, Claudia Folli, Davide Cavazzini, Giovanni Sartori, and Rodolfo Berni
Fluorometric Titration of the CRABPs
Andrew W. Norris and Ellen Li
Expression and Mutagenesis of Retinol-Binding Protein
Manickavasagam Sundaram, Asipu Sivaprasadarao, and John B. C. Findlay
Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor
Asipu Sivaprasadarao, Manickavasagam Sundaram, and John B. C. Findlay
Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis
Robert S. Jamison, Marcia E. Newcomer, and David E. Ong
Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein
Noa Noy
Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression
Paul Nugent and Robert M. Greene
Part III. Nuclear Retinoid Receptors
Preparation of Polyclonal Antibodies to Retinoid Receptors
Jacqueline A. Dyck and Vickie J. LaMorte
Detection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding Immunoprecipitation Techniques
Elizabeth A. Allegretto
Nonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue Sections
Xiaochun Xu and Reuben Lotan
In Situ Hybridization with S-Labeled Probes for Retinoid Receptors
Karen Niederreither and Pascal Dollé
Isolation of Retinoid Receptors from Mammalian Cells
Ann K. Daly and Christopher P. F. Redfern
Analysis of Retinoid Receptor Phosphorylation
Ali Tahayato, Christophe Rachez, Pierre Formstecher, and Philippe Lefebvre
Photoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Techniqu
Yuichi Hashimoto and Toru Sasaki
PCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RARa Fusion Proteins
Arthur Zelent
RT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL)
David Grimwade, Stephen Langabeer, Kathy Howe, and Ellen Solomon
A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors
Paul N. MacDonald
Gel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based Selection
Myriam I. Baes and Peter E. Declercq
Identification and Cloning of RA-Regulated Genes by mRNA Differential Display
Jay A. White and Martin Petkovich
Gene Targeting of Retinoid Receptors
David Lohnes
Index