Developmental Biology Protocols: Volume I

Part I. Introduction. Developmental Biology Protocols: Overview I, Rocky S. Tuan and Cecilia W. Lo. Part II. Systems: Production, Culture, and Storage. Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies, Christopher J. Lowe and Gregory A. Wray. Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins, James A. Coffman and Patrick S. Leahy. The Chick Embryo as a Model System for Analyzing Mechanisms of Development, Diana K. Darnell and Gary C. Schoenwolf. Culture of Avian Embryos, Diana K. Darnell and Gary C. Schoenwolf. Ex Ovo Culture of Avian Embryos, Tamao Ono. Culture of Preimplantation Mouse Embryos, Adam S. Doherty and Richard M. Schultz. In Vitro Culture of Rodent Embryos During Early Postimplantation Period, Masahiko Fujinaga. Cryopreservation of Mouse Embryos, Jean Richa. Part III. Developmental Pattern and Morphogenesis. Studying Head and Brain Development in Drosophila, Robert Finkelstein. Bioassays of Inductive Interactions in Amphibian Development, Takashi Ariizumi, Kazuhiro Takano, Makoto Asashima, and George M. Malacinski. Gastrulation and Early Mesodermal Patterning in Vertebrates, Gary C. Schoenwolf and Jodi L. Smith. Craniofacial Development of Avian and Rodent Embryos, Brian K. Hall and Tom Miyake. Examination of the Axial Skeleton of Fetal Rodents, Michael G. Narotsky and John M. Rogers. Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach, B. Rush Waller III and Andy Wessels. Part IV. Embryo Structure and Function. Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos, Kersti K. Linask and Takeshi Tsuda. Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos, Carolyn A. Larabell. Whole-Mount Immunolabeling of Embryos by Microinjection: Increased Detection Levels of Extracellular and Cell Surface Epitopes, Charles D. Little and Christopher J. Drake. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos, Robert M. Zucker, E. Sidney Hunter III, and John M. Rogers. Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy, Robert M. Zucker and John M. Rogers. Magnetic Resonance Imaging Analysis of Embryos, Bradley R. Smith. Optical Coherence Tomography Imaging in Developmental Biology, Stephen A. Boppart, Mark E. Brezinski, and James G. Fujimoto. Ultrasound Backscatter Microscopy of Mouse Embryos, Daniel H. Turnbull. Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function, Kersti K. Linask and James C. Huhta. Calcium Imaging and Cell-Cell Signaling, Diane C. Slusarski and Victor G. Corces. Acquisition, Display, and Analysis of Digital Three-Dimensional and Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications, Charles F. Thomas and John G. White. Part V. Cell Lineage Analysis. Cell Lineage Analysis: Applications of Green Fluorescent Protein, Magdalena Zernicka-Goetz and Jonathon Pines. Cell Lineage Analysis: X-Inactivation Mosaics, Seong-SengTan, Leanne Godinho, and Patrick P. L. Tam. Retroviral Cell Lineage Analysis in the Developing Chick Heart, Robert G. Gourdie, Gang Cheng, Robert P. Thompson, and Takashi Mikawa. Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos, Diana K. Darnell, Virginio Garcia-Martinez, Carmen Lopez-Sanchez, Shipeng Yuan, and Gary C. Schoenwolf. Cell Lineage Analysis: Videomicroscopy Techniques, Paul J. Heid and Jeff Hardin. Cell Lineage Analysis in Xenopus Embryos, Sally A. Moody. Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryos, David J. Kozlowski and Eric S. Weinberg. Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo, Dazhong Sun, C. May Griffith, and Elizabeth D. Hay. Part VI. Chimeras. Transplantation Chimeras: Use in Analyzing Mechanisms