Calcium-Binding Protein Protocols: Volume 1: Reviews and Case Studies

Part I. Introduction and ReviewsCalcium-Binding ProteinsHans J. Vogel, Richard D. Brokx, and Hui OuyangCalciumRobert J. P. WilliamsCrystal Structure of Calpain and Insights into Ca2+-Dependent ActivationZongchao Jia, Christopher M. Hosfield, Peter L. Davies, and John S. ElceThe Multifunctional S100 Protein FamilyClaus W. HeizmannCa2+ Binding to Proteins Containing g-Carboxyglutamic Acid ResiduesEgon PerssonThe Caseins of Milk as Calcium-Binding ProteinsHarold M. Farrell, Jr., Thomas F. Kumosinski, Edyth L. Malin, and Eleanor M. BrownPart II. Calcium-Binding Proteins: Case StudiesPreparation of Recombinant Plant Calmodulin IsoformsRaymond E. ZielinskiIsolation of Recombinant Cardiac Troponin CJohn A. Putkey and Wen LiuSkeletal Muscle Troponin C: Expression and Purification of the Recombinant Intact Protein and Its Isolated N- and C-Domain FragmentsJoyce R. Pearlstone and Lawrence B. SmilliePurification of Recombinant Calbindin D9kEva ThulinS100 Proteins: From Purification to FunctionsJean Christophe Deloulme, Gaëlh Ouengue Mbele, and Jacques BaudierCadherinsJean-René Alattia, Kit I. Tong, Masatoshi Takeichi, and Mitsuhiko Ikuraa-Lactalbumin and (Calcium-Binding) LysozymeKatsutoshi NittaRecombinant Annexin II TetramerHyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, and David M. WaismanPurification and Characterization of ALG-2: A Novel Apoptosis-Linked Ca2+-Binding ProteinMingjie Zhang and Kevin W.-H. LoCrystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain V1 of CalpainMiroslaw Cygler, Pawel Grochulski, and Helen BlanchardNeurocalcin: Role in Neuronal SignalingSenadhi Vijay-Kumar and Vinod D.KumarCrystallization and Structure-Function of CalsequestrinChulHee Kang, William R. Trumble, and A. Keith DunkerUse of Fluorescence Resonance Energy Transfer to Monitor Ca2+-Triggered Membrane Docking of C2 DomainsEric A. Nalefski and Joseph J. FalkeCa2+-Binding Mode of the C2A-Domain of SynaptotagminJosep Rizo, Josep Ubach, and Jesús GarcíaStudy of Calcineurin Structure by Limited ProteolysisSeun-Ah Yang and Claude Klee