Antibody Phage Display: Methods and Protocols

Overview of Antibody Phage Display Technology and its Applications
Hennie R. Hoogenboom
Standard Protocols for the Construction of Fab Libraries
Michelle A. Clark
Standard Protocols for the Construction of scFv Libraries
Simon Lennard
Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice
Philippa M. O'Brien and Robert Aitken
Construction of Large Naive Fab Libraries
Hans J. W. de Haard
Construction of Polyclonal Antibody Libraries Using Phage Display
Jacqueline Sharon, Seshi R. Sompuram, Chiou-Ying Yang, Brent R. Williams and Stefanie Sarantopoulos
Antigen-Driven Stimulation of B Lymphocytes In Vitro
Zhiwei Hu
The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion
Ruud M. T. de Wildt and René M. A. Hoet
Panning of Antibody Phage Display Libraries: Standard Protocols
David W. J. Coomber
Selection of Antibodies Against Biotinylated Antigens
Patrick Chames, Hennie R. Hoogenboom and Paula Henderikx
Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries
Keith A. Charlton and Andrew J. Porter Blocking Immunodominant Epitopes by Competitive Deselection
Roberto Burioni
Rescue of a Broader Range of Antibodies Using an Epitope-Masking Strategy
Henrik J. Ditzel
Screening of Phage-Expressed Antibody Libraries by Capture Lift
Jeffry D. Watkins
Antibody-Guided Selection Using Capture Sandwich ELISA
Kunihiko Itoh and Toshio Suzuki
Proximity-Guided (ProxiMol) Antibody Selection
Jane Osbourn
Isolation of Human mAbs Using Guided Selection With Mouse mAbs
Mariangela Figini and Silvana Canevari
Selecting Antibody to Cell Surface Antigens Using Magnetic SortingTechniques
Don L. Siegel
Isolation of Human Tumor-Associated Cell Surface Antigen binding scFv Fragments
E. Jacintha Noronha, Xinhui Wang and Soldano Ferrone
Subtractive Isolation of Single Chain Antibodies Using Tissue Fragments
Katarina Radosevic and Willem van Ewijk
Selection of Antibodies Based on Antibody Kinetic Binding Properties
Ann-Christin Malmborg, Nina Nilsson and Mats Ohlin
Selection of Functional Antibodies on the Basis of Valency
Manuela Zaccolo
Two-Step Strategy for Alteration of Immunoglobulin Specificity by in vitro Mutagenesis
Yoshitaka Iba, Chie Miyazaki and Yoshikazu Kurosawa
Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement
Partha S. Chowdhury
Use of Error-Prone PCR for the Modification of Single-Chain Fv Fragments
Pierre Martineau
Use of E. coli Mutator Cells to Mature Antibodies
Robert Irving, Gregory Coia, Anna Raicevic and Peter J. Hudson
Chain Shuffling to Modify the Properties of Recombinant Immunoglobulins
Johan Lantto, Pernilla Jirholt, Yvelise Barrios and Mats Ohlin
Generation of Bispecific and Tandem Diabodies
Sergey M. Kipriyanov
High Level Periplasmic Expression and Purification of scFv Fragments
Sergey M. Kipriyanov
Periplasmic Expression and Purification of Recombinant Fab Fragments
Robert L. Raffai
Expression of Antibody Fragments in Pichia pastoris
Philipp Holliger
Expression of VHH Antibody Fragments in Saccharomyces Cerevisiae
J. Marcel van der Vaart
Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments
Pascale A. Cohen
Expression of scFvs and scFv-Fusion Proteins in Eukaryotic Cells
Michelle de Graaf, Ida van der Muelen-Muileman and Hidde J. Haisma
Expression of Antibody Fabs Fragments and