Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols

Part I. Techniques for Histological IdentificationCytochemical Localization of H2O2 in Biological TissuesE. Ann Ellis and Maria B. GrantLocalization of Intracellular Lipid Peroxides Using Tetramethylbenzidine Reaction for Transmission Electron MicroscopyE. Ann Ellis, Shigehiro Iwabuchi, Don Samuelson, and Donald ArmstrongThe Immunohistochemical Localization of Glutathione PeroxidaseKiyoshi Akeo, Tadahisa Hiramitsu, and Keiichi Watanabe4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE AntibodyHideyuki J. Majima, Takako Nakanishi-Ueda, and Toshihiko OzawaImmunohistochemical Detection of Protein OxidationJürgen Frank, Alfonso Pompella, and Hans K. BiesalskiIndirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated CellsAlfonso Pompella, Silvia Dominici, Jürgen Frank, and Hans K. BiesalskiPart II. Techniques for Subcellular LocalizationMethods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts(AGE Receptors)Paul J. ThornalleyMeasurement of Blue Fluorescence as a Protein Marker for Oxidized MembranesKiyomi KikugawaX-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative StressR. Preston Mason and Robert F. JacobDetection of Estrogen Receptor by In Situ HybridizationKaori Kobayashi and Hiroshi KobayashiTumor Necrosis Factor-a Quantification and Expression by In Situ HybridizationTracey A. Ignatowski and Robert N. SpenglerPart III. Techniques for Molecular BiologyTranslocation of p47phox and Activation of NADPH Oxidase in Mononuclear CellsAhmad Aljada, Husam Ghanim, and Paresh DandonaActivation of Nuclear Factor-kB (NF-kB) in Mononuclear Cells (MNC)Ahmad Aljada, Husam Ghanim, and PareshDandonaAssay of Intracellular Hydrogen Peroxide Generation in Activated Individual Neutrophils by Flow CytometryYoshikazu Ito and David A. LipschitzDNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)Gieri Camenisch, Roland H. Wenger, and Max GassmannAnalysis of Heat-Shock Transcription Factor and Element-Binding ActivityYong J. LeeSlot-Blot Hybridization in Studying Gene Expression During Oxygen DeprivationEnbo Ma and Gabriel G. HaddadAn Expression System for a Transporter of Iron and Other MetalsMichael D. Garrick and Kevin G. DolanAnalysis of Gene Expression Following Oxidative StressDana R. Crawford, Toshihide Suzuki, Jan Sesay, and Kelvin J. A. DaviesMeasurement of Immunoglobulin G Oxidation by Western-Blot AnalysisAndrew Chow, Shahid Ahmed, Larrisa Chaplia, and Joseph MattanaThioredoxin and Redox Regulation of the Nuclear ReceptorYuichi Makino, Kensaku Okamoto, and Hirotoshi TanakaAnalysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNAXin Gen Lei and Wen-Hsing ChengExpression of Human Phospholipid Hydroperoxide Glutathione PeroxidaseKunio Yagi, Sadaaki Komura, and Nobuko OhishiPart IV. Techniques for In Vivo/Ex Vivo/In Vitro ApplicationsSuperoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence MethodTakashi Sakurai and Susumu TerakawaIn Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance SpectroscopyDavid M. Hall and Garry R. BuettnerSpectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHzJürgen Fuchs, Norbert Groth, and Thomas HerrlingIn Vivo/In Situ Detection of Nitric Oxide Using Low-Frequency EPR SpectroscopyPei Tsai, Supatra Porasuphatana, Howard J. Halpern,