Peptide Nucleic Acids: Methods and Protocols

Part I. IntroductionPNA TechnologyPeter E. NielsenPart II. ChemistrySolid Phase Synthesis of PNA OligomersFrederik BeckSynthesis of PNA-Peptide ConjugatesSatish Kumar Awasthi and Peter E. NielsenParallel Synthesis of PNA-Peptide Conjugate LibrariesSatish Kumar Awasthi and Peter E. NielsenThermodynamics of PNA Interactions with DNA and RNATommi Ratilainen and Bengt NordénPart III. PNA TechnologyMeasurement of PNA Binding to Double-Stranded DNAThomas Bentin, Georg I. Hansen, and Peter E. NielsenPNA-Mediated Immobilization of Supercoiled DNAThomas Bentin and Peter E. NielsenPNA Openers and Their ApplicationsVadim V. Demidov and Maxim D. Frank-KamenetskiiColorimetric Detection of PNA-DNA Hybridization Using Cyanine DyesMiaomiao Wang and Bruce A. ArmitagePart IV. DiagnosticsPNA-Mediated PCR Clamping: Applications and MethodsDeborah G. Murdock and Douglas C. WallacePNA Clamping Techniques for the Determination of Oncogene MutationsMarcus Schuermann and Moira Behn. PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic AnalysisBrett Williams, Henrik Stender, and James M. CoullDetection of Point Mutations Using PNA-Containing Electrophoresis MatricesGabor L. IgloiPart V. Antisense and AntigeneLipid-Mediated Introduction of Peptide Nucleic Acids Into CellsDwaine A. Braasch and David R. CoreySynthesis of Cell-Penetrating Peptide-PNA ConstructsMargus Pooga, Ursel Soomets, Tamas Bartfai, and Ülo LangelAntisense Inhibition of Bacterial Gene Expression and Cell GrowthLiam GoodIn Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement LoopsNiels Erik Møllegaard and Peter E. NielsenPart VI.Animal StudiesIn Vitro and in Vivo Studies on Pharmacokinetics and Metabolism of PNA Constructs in RodentsEdward KristensenIndex