HPLC of Peptides and Proteins: Methods and Protocols

Part I. Modes of HPLC HPLC of Peptides and Proteins: Basic Theory and Methodology Marie-Isabel Aguilar Reversed-Phase High-Performance Liquid Chromatography Marie-Isabel Aguilar Ion-Exchange Chromatography Peter Stanton High-Performance Hydrophobic Interaction Chromatography Kálmán Benedek Gel Filtration Chromatography Peter Stanton Hydrophilic Interaction Chromatography Herbert Lindner and Wilfried Helliger Immobilized Metal Ion Affinity Chromatography of Proteins Michael Zachariou Immunoaffinity Chromatography of Proteins Stuart R. Gallant Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins Tun-Li Shen and Kathleen R. Noon Part II. Preparative Methodologies Capillary Separations Montserrat Carrascal and Joaquin Abian Micropreparative HPLC of Peptides and Proteins Edouard C. Nice and Marie-Isabel Aguilar Multidimensional HPLC Purification of Proteins Edouard C. Nice and Marie-Isabel Aguilar Analytical High-Performance Liquid Chromatography Kálmán Benedek Prep/Semiprep Separations of Peptides Denis B. Scanlon and James Finlayson Large-Scale Protein Chromatography Joseph Bertolini, Peter Gomme, and Patrick Thomas Part III. Applications Proteolytic Peptide Mapping, Peter Højrup Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation Martin R. Larsen Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry Nana Kawasaki, Miyako Ohta, Satsuki Itoh, and Takao Hayakawa HPLC in the Analysis of Peptide Metabolism Rebecca A. Lew Isolation and Characterization of Naturally ProcessedMHC-Bound Peptides From the Surface of Antigen-Presenting Cells Anthony W. Purcell Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques David Wilson and Paul Alewood HPLC and Mass Spectrometry of Intrinsic Membrane Proteins Julian P. Whitelegge IgG Purification Maree S. Powell and Bruce D. Wines DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions Malcolm Buckle Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC Jordi Gómez-Ramirez, Isaac Blanco, and Jordi Ortiz Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry Jean-Louis Aubagnac, Christine Enjalbal, Jean Martinez, Pierre Sanchez, and Gilles Subra Proteome Analysis Matthew J. Powell and Aaron T. Timperman