Plant Cold Acclimation: Methods and Protocols

1. Introduction: Plant Cold Acclimation and Winter Survival

            Dirk K. Hincha and Ellen Zuther

2. Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays

            Anja Thalhammer, Majken Pagter, Dirk K. Hincha, and Ellen Zuther

3. Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements

            Andrey V. Malyshev, Ilka Beil, and Juergen Kreyling

4. Infrared Thermal Analysis of Plant Freezing Processes

            Gilbert Neuner and Edith Kuprian

5. Conducting Field Trials for Frost Tolerance Breeding in Cereals

            Luigi Cattivelli and Cristina Crosatti

6. A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes

            Annick Bertrand, Annie Claessens, Josée Bourassa, Solen Rocher, and Vern Baron

7. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

            Evelyne Téoulé and Carine Géry

8. Identification of Arabidopsis Mutants with Altered Freezing Tolerance

            Carlos Perea-Resa, Rafael Catalá, and Julio Salinas

9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

            Seizo Fujikawa and Keita Endoh

10. Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue

            David P. Livingston III and Tan D. Tuong

11. Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography

            Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, and Karen K. Tanino

12. Proteomic Approaches to Identify Proteins Responsive to Cold Stress

            Anna M. Jozefowicz, Stefanie Döll, and Hans-Peter Mock

13. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins

            Kamal Md. Mostafa, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, and Matsuo Uemura

14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

            Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura, and Ruth Welti

15. Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

            Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle, Jessica Alpers, Olga Beine-Golovchuk, and Joachim Kopka

16. Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation

            Andras Bittner, Thomas Griebel, Jörn van Buer, Ilona Juszczak-Debosz, and Margarete Baier

17. Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation

            Daisuke Takahashi, Ellen Zuther, and Dirk K. Hincha

18. Subcellular Compartmentation of Metabolites Involved in Cold Acclimation

            Imke I. Hoermiller, Thomas Nägele, and Arnd G. Heyer

19. Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime

            Lisa Fürtauer and Thomas Nägele

20. Characterization of Ice Binding Proteins from Sea-Ice Microalgae

            Maddalena Bayer-Giraldi, EonSeon Jin, and Peter W. Wilson

21. Isolation and Characterization of Ice-Binding Proteins from Higher Plants

            Melissa Bredow, Heather E. Tomalty, Laurie A. Graham, Audrey K. Gruneberg, Adam J. Middleton, Barbara Vanderbeld, Peter L. Davies, and Virginia K. Walker