Cardiac Regeneration: Methods and Protocols

Part I: Cardiac Injury Models

1. Myocardial Infarction Techniques in Adult Mice

            Elad Bassat, Dahlia Perez, and Eldad Tzahor

2. Apical Resection and Cryoinjury in Neonatal Mouse Heart

            Hua Shen, Ali Darehzereshki, Henry M. Sucov, and Ching-Ling Lien

3. Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice

            Tilman Ziegler, Karl-Ludwig Laugwitz, and Christian Kupatt

4. Myocardial Infarction in Pigs

            Andrea Bähr, Nadja Hornaschewitz, and Christian Kupatt

5. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish

            Ines J. Marques, Andrés Sanz-Morejón, and Nadia Mercader

6. Cardiac Resection Injury in Zebrafish

            Delicia Z. Sheng, Dawei Zheng, and Kazu Kikuchi

7. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish

            Fei Sun, Adam R. Shoffner, and Kenneth D. Poss

8. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration

            Yijen L. Wu

Part II: Ex Vivo and In Vivo Approaches

9. Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes

            Marina Leone and Felix B. Engel

10. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes

            Lu Han, Jocelyn Mich-Basso, and Bernhard Kühn

11. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells

            Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, and Ravi Karra

12. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells

            Jun Xu, Li Wang, Jiandong Liu, and Li Qian

13. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs

            Abbigail Helfer and Nenad Bursac

14. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells

            Ling Gao and Jianyi Zhang

15. Isolation[SL1] [DC2]  and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts

            Honghai Liu, Kevin Bersell, and Bernhard Kühn

16. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish

            Sierra Duca and Jingli Cao

17. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters

            Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, and Sean M. Wu

Part III: Visualizing and Manipulating Heart Regeneration

18. In Vivo Clonal Analysis of Cardiomyocytes

            Ngoc B. Nguyen, G. Esteban Fernandez, Yichen Ding, Tzung Hsiai, and Reza Ardehali

19. High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry

            Frank Gyngard, Louise Trakimas, and Matthew L. Steinhauser

20. AAV Gene Transfer to the Heart

            Suya Wang, Yuxuan Guo, and William T. Pu

21. In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy

            Nishat Sultana, Mohammad Tofael Kabir Sharkar, Yoav Hadas, Elena Chepurko, and Lior Zangi

22. Generation and Manipulation of Exosomes

            Shiqi Hu, Li Qiao, and Ke Cheng

23. Epigenetic Assays in Purified Cardiomyocyte Nuclei

            Matthew C. Hill and James F. Martin

24. Genetic Lineage Tracing of Non-Cardiomyocytes in Mice

            Zhongming Chen and Jop H. van Berlo

25. Experimental Hypoxia as a Model for Cardiac Regeneration in Mice

            Yuji Nakada and Hesham A. Sadek

 [SL1]

 [DC2]