Kupffer Cells: Methods and Protocols

1. Kupffer Cell and Hepatocytes Isolation by Gradient Centrifugation from a Single Mouse Liver

2.  Kupffer Cell Isolation from Human Biopsies

Cecilia Morgantini

3. Liver Macrophage Isolation by Flow Cytometry Sorting

André Sulen

4. Kupffer Cell Protein Isolation and Detection by Western Blot

Valerio Azzimato

5. Kupffer Cell mRNA Sequencing

Connie Xu and Emelie Barreby

6. Kupffer Cell and Monocyte-derived Macrophage Identification by Immunofluorescence on FFPE Mouse Liver Sections

Adrien Guillot, Chloé Buch, and Tony Jourdan

7. Sample Preparation of Optically Cleared Liver Tissue to Identify Liver Macrophages using 3D Microscopy

Otto Strauss and Niklas K. Björkström

8. Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer cells in mouse liver

Michaela Tencerova

9. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

Qiushi Tang, Gwladys Gernoux, Yangfun Cheng, Terence Flotte, and Christian Mueller

10. Kupffer Cell Characterization by Mass Cytometry

Camille Blériot, Shamin Li, Muhammad Faris Bin Mohd Kairi, Evan Newell, and Florent Ginhoux

11. Enzymatic Quantification of Liver Lipids after Folch Extraction

Mirko E. Minniti, Osman Ahmed, and Matteo Pedrelli

12. In vivo Investigation of High Fat Diet Induced Hepatic Lipid Dysfunctions

Marion Korach-André

13. An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis

Bader Zarrouki and Jeremie Boucher

14. Generating Hepatocellular Carcinoma in Mice By Expressing N-Ras^G12V Accompanied with Trp53 Silence Using A Transposon-based System Delivered via Hydrodynamic Ail Vein Injection

Sijia Yu and Santiago Vernia

15. Mouse Model of Alcoholic Steatohepatitis

Adeline Bertola