Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols

1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)

            Linda Z. Holland and Guang Li

2. Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata

            Ryusaku Deguchi and Takeshi Nakano

3. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae

            Naoyuki Murabe, Ei-ichi Okumura, Kazuyoshi Chiba, Enako Hosoda, Susumu Ikegami, and Takeo Kishimoto

4. Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis

            Aldine R. Amiel and Eric Röttinger

5. Staining and Tracking Methods for Studying Sponge Cell Dynamics

            Carole Borchiellini, Sandie M. Degnan, Emilie Le Goff, Caroline Rocher, Amélie Vernale, Stephen Baghdiguian, Nina Séjourné, Florent Marschal, André Le Bivic, Nelly Godefroy, Bernard M. Degnan, and Emmanuelle Renard

6. Microscopy Studies of Placozoans

            Carolyn L. Smith, Tatiana D. Mayorova, Christine A. Winters, Thomas S. Reese, Sally P. Leys, and Andreas Heyland

7. Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata

            Lauren Bates, Emily Wiseman, Jamie Kitson, and David J. Carroll

8. Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging

            Stephen A. Stricker

9. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization

            Emily Wiseman, Lauren Bates, and David J. Carroll

10. Quantifying Cell Proliferation During Regeneration of Aquatic Worms

            Eduardo E. Zattara and B. Duygu Özpolat

11. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis

            Laura Fierro-Constain, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Sejourné, Carole Borchiellini, and Emmanuelle Renard

12. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond

            Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, and Gary M. Wessel

13. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation

            Alex McDougall, Celine Hebras, Isa Gomes, and Remi Dumollard

14. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis

            Jacob F. Warner and Eric Röttinger

15. RNA Interference on Regenerating Holothurian Gut Tissues

            Miosotis Alicea-Delgado, Samir A. Bello-Melo, and José E. García-Arrarás

16. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos

            Marta Silvia Magri, Danila Voronov, Jovana Ran_elovic, Claudia Cuomo, Jose Luis Gómez-Skarmeta, and Maria I. Arnone

17. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase

            Sonoko Kinjo, Masato Kiyomoto, Takashi Yamamoto, Kazuho Ikeo, and Shunsuke Yaguchi

18. Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity

            Julia Gauberg, Adriano Senatore, and Andreas Heyland

19. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna

            Joseph L. Sevigny, Jon L. Norenburg, and Francesca Leasi