Phosphoinositides: Methods and Protocols

1. Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling

            Noah Steinfeld, Sai Srinivas Panapakkam Giridharan, Emily J. Kauffman, and Lois S. Weisman

2. Label-Free Quantification of Phosphoinositides in Drosophila by Mass Spectrometry

            Avishek Ghosh and Padinjat Raghu

3. Profiling of Phosphoinositide Molecular Species in Resting or Activated Human or Mouse Platelets by a LC-MS Method

            Gaëtan Chicanne, Justine Bertrand-Michel, Julien Viaud, Karim Hnia, Jonathan Clark, and Bernard Payrastre

4. Quantification of Genetically-Encoded Lipid Biosensors

            Rachel C. Wills, Jonathan Pacheco, and Gerald R.V. Hammond

5. Detection of Plasma Membrane Phosphoinositide Dynamics Using Genetically-Encoded Fluorescent Protein Probes

            Rebecca Cabral-Dias, Yasmin Awadeh, Roberto J. Botelho, and Costin N. Antonescu

6. Imaging the Nanoscale Distribution of Phosphoinositides in the Cell Plasma Membrane with Single-Molecule Localization Super-Resolution Microscopy

            Fan Fan, Chen Ji, and Xuelin Lou

7. Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates

            Jonathan Pacheco, Rachel C. Wills, and Gerald R.V. Hammond

8. A Real-Time Phosphatidylinositol 4-Phosphate 5-Kinase Assay Using Fluorescence Spectroscopy

            Taki Nishimura

9. Assessing In Situ Phosphoinositide-Protein Interactions through Fluorescence Proximity Ligation Assay in Cultured Cells

            Mo Chen, Hudson T. Horn, Tianmu Wen, Vincent L. Cryns, and Richard A. Anderson

10. Characterization of Protein-Phospholipid/Membrane Interactions Using a "Membrane-on-a-Chip" Microfluidic System

            Calvin Yeager, Djoshkun Shengjuler, Simou Sun, Paul S. Cremer, and Craig E. Cameron

11. Exploring Phosphoinositide Binding Using Native Mass Spectrometry

            Julian Bender and Carla Schmidt

12. Liposome-Based Methods to Study Protein-Phosphoinositide Interaction

            Mélanie Mansat, Mélanie Picot, Gaëtan Chicanne, Virginie Nahoum, Frédérique Gaits-Iacovoni, Bernard Payrastre, Karim Hnia, and Julien Viaud

13. Liposome-Based Methods to Study GTPases Activation by Phosphoinositides

            Julien Viaud, Laurie Ceccato, Bernard Payrastre, and Frédérique Gaits-Iacovoni

14. Liposome Co-Sedimentation and Co-Flotation Assays to Study Lipid-Protein Interactions

            Yosuke Senju, Pekka Lappalainen, and Hongxia Zhao

15. Characterization of PROPPIN-Phosphoinositide Binding by Stopped-Flow Fluorescence Spectroscopy

            Ángel Pérez-Lara and Reinhard Jahn

16. Fluorescence Assays to Study Membrane Penetration of Proteins

            Yosuke Senju and Hongxia Zhao

17. Fluorogenic XY-69 in Lipid Vesicles for Measuring Activity of Phospholipase C Isozymes

            Adam J. Carr, Edhriz Siraliev-Perez, Weigang Huang, John Sondek, and Qisheng Zhang