PDZ Mediated Interactions: Methods and Protocols

Preface...
Table of Contents...
Contributing Authors...

1.Identification of PDZ Interactions by Yeast Two-Hybrid 
Monica Castro-Cruz, Marta Monserrat-Gomez, Jean-Paul Borg, Pascale Zimmermann, and Eric Bailly 

2.Identification of PDZ Interactions by Affinity Purification and Mass Spectrometry Analysis
Avais M. Daulat , Stéphane Audebert, Mônica Wagner, Luc Camoin, and Jean-Paul Borg

3.Identification of PDZ Interactions by Proteomic Peptide Phage Display
Susanne Lüchow, Gustav Sundell, and Ylva Ivarsson

4.A Computational Protocol to Analyze PDZ/PBM Affinity Data Obtained by High-Throughput Holdup Assay
Pau Jané, Lionel Chiron, Goran Bich, Gilles Travé, and Yves Nominé

5.Study of PDZ-Peptide and PDZ-Lipid Interactions by Surface Plasmon Resonance/BIAcore
Pascale Zimmermann and Antonio Luis Egea-Jimenez

6.PDZ Sample Quality Assessment by Biochemical and Biophysical Characterizations
Célia Caillet-Saguy, Sébastien Brûlé, Nicolas Wolff, and Bertrand Raynal

7.Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module
Janesha C. Maddumage, Bryce Z. Stewart, Patrick O. Humbert, and Marc Kvansakul

8.A Fluorescence-Based Assay to Determine PDZ-Ligand Binding Thermodynamics
Young Joo Sun and Ernesto J. Fuentes

9.Unveiling the Folding Mechanism of PDZ Domains
Candice Gautier and Stefano Gianni 

10.Development of Peptide-Based PDZ Domain Inhibitors
Dominik J. Essig, Javier R. Balboa, and Kristian Strømgaard

11.Dynamic Control of Signaling by Phosphorylation of PDZ Binding Motifs
Márton A. Simon and László Nyitray  

12.Chemical Synthesis of PDZ Domains
Christin Kossmann, Sana Ma, Louise S. Clemmensen, and Kristian Strømgaard

13.Viral PDZ Binding Motifs Influence Cell Behavior through the Interaction with Cellular Proteins Containing PDZ Domains
Carlos Castaño-Rodriguez, Jose M. Honrubia, Javier Gutiérrez-Álvarez, Isabel Sola, and Luis Enjuanes

14.Computational Design of PDZ:peptide Binding 
Nicolas Panel, Francesco Villa, Vaitea Opuu, David Mignon, and Thomas Simonson

15.Mechanoregulation of PDZ Proteins, An Emerging Function
Elsa Bazellières and André Le Bivic

16.  Rational Design of PDZ Domain Inhibitors: Discovery of Small Organic Compounds Targeting PDZ Domains
Laurent Hoffer, Philippe Roche, and Xavier Morelli

Subject Index List...