Mass Spectrometry of Glycoproteins: Methods and Protocols

Preface...Table of Contents...Contributing Authors...

1.Glycosylation of Therapeutic Proteins: A Critical Quality AttributeArnaud Delobel
2.Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass SpectrometryDan Bach Kristensen, Trine Meiborg Sloth, Martin Ørgaard, and Pernille Foged Jensen
3.Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryEstela Giménez, Montserrat Mancera-Arteu, Fernando Benavente, and Victoria Sanz-Nebot
4.Fc Glycosylation Characterization of Human Immunoglobulins G using Immunocapture and LC-MSYosra Helali, Shilpee Sharma, Marie Vandeput, Dansala Welba, Pierre Van Antwerpen, Arnaud Marchant, and Cédric Delporte 
5.Fast Afucosylation Profiling of Glyco-Engineered Antibody Subunits by Middle Up Mass SpectrometryElsa Wagner-Rousset, Olivier Colas, Stéphane Chenu, Yannis-Nicolas François, Davy Guillarme, Sarah Cianferani, Yury O. Tsybin, Jonathan Sjögren, Arnaud Delobel, and Alain Beck
6.Characterization of Glycosylated Proteins at Subunit Level by HILIC/MSValentina D'Atri and Davy Guillarme
7.Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis - Mass Spectrometry Coupling (CE-MS)Josiane Saadé, Michael Biacchi, Jérémie Giorgetti, Antony Lechner, Alain Beck, Emmanuelle Leize-Wagner, and Yannis-Nicolas François
8.Enrichment of Intact Glycopeptides using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction ChromatographyAbel Bermudez and Sharon J. Pitteri
9.In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC -SPE and LC-MS/MSMyung Jin Oh, Youngsuk Seo, Unyong Kim, and Hyun Joo An
10.Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSEKevin Canis, Estelle Garénaux, and Jean-François Boe
11.Mapping O-glycosylation Sites using OpeRATOR and LC-MSMaria Nordgren, Andreas Nägeli, Helén Nyhlén, and Jonathan Sjögren
12.Site-Specific O-Glycosylation Analysis by Liquid Chromatography/Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional DissociationNoritaka Hashii and Junya Suzuki
13.Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS after Derivatization with Fluorescent AnthranilamideClaire I. Butré, Eric Largy, and Arnaud Delobel
14.Evaluating N-glycosylation of a Therapeutic Monoclonal Antibody using UHPLC-FLR-MS with RapiFluor-MS LabelingRosie Upton, James Duffy, Sam Clawson, and David Firth
15.Routine Analysis of N-glycans using Liquid Chromatography Coupled to Routine Mass DetectionXimo Zhang, Vithiya Vimalraj, and Mitul Patel
16.Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals Sonja Schneider, Edgar Naegele, and Sonja Krieger
17.Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS after Derivatization with Fluorescent AnthranilamideClaire I. Butré, Eric Largy, Fabrice Cantais, and Arnaud Delobel
18.Linkage Analysis of Oligo- and Polysaccharides: A TutorialIan Black, Christian Heiss, Russell W. Carlson, and Parastoo Azadi
19.Use of Exoglycosidases for the Structural Characterization of GlycansBeth McLeod, Paula Magnelli, and Xiaofeng Shi
20.Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography-Mass Spectrometry (LC-MS)Byeong Gwan Cho, Alireza Banazadeh, Wenjing Peng, Jingfu Zhao, Mona Goli, Sakshi Gautam, Ahmed Hussein, and Yehia Mechref
21.Optimization of Multiple Glycosidase and Chemical Stabilization Strategies for N-glycan Isomer Detection by Mass Spectrometry Imaging in Formalin-Fixed, Paraffin-Embedded TissuesConnor A. West, Xiaowei Lu, Grace Grimsley, Kim Norris-Caneda, Anand S.  Mehta, Peggi M. Angel, and Richard R. Drake
22.Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MSJesús S. Aguilar Díaz de león and Chad R. Borges
23.Array-Based N-Glycan Profiling of Cells in CulturePeggi M. Angel, Anand S. Mehta, and Richard R. Drake
24.Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multi-Angle Light ScatteringKathryn Hastie, Vamseedhar Rayaprolu, and Erica Ollmann Saphire
25.Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative AssessmentAllison Derenne, Kheiro-Mouna Derfoufi, Ben Cowper, Cédric Delporte, Claire I. Butré, and Erik Goormaghtigh
26.Deploying Mass Spectrometric Data Analysis in the Amazon AWS Cloud Computing EnvironmentJonathan E. Katz
Subject Index List...