Plant Photomorphogenesis: Methods and Protocols

1. Setting up an Arabidopsis LED Culture Module that Simulates Plant Neighbor Proximity

            Carlos D. Crocco

2. Photobody Detection Using Immunofluorescence and Super-Resolution Imaging in Arabidopsis

            Giorgio Perrella, Anna Zioutopoulou, Andrew Hamilton, and Eirini Kaiserli

3. Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis

            Yetkin Çaka Ince and Vinicius Costa Galvão

4. Isolation of UVR8 Protein Complexes

            Yan Liu and Xi Huang

5. Phenotypic Study of Photo-Morphogenesis in Arabidopsis Seedlings

            Chuanwei Yang, Famin Xie, and Lin Li

6. Experimental Procedures for Studying Skotomorphogenesis in Arabidopsis thaliana

            Huanhuan Jin, Hong Li, and Ziqiang Zhu

7. Global Identification for Targets of Circadian Transcription Factors in Arabidopsis and Rice Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq)

            Shuxuan Xu, Jing Huang, Jian Jin, and Wei Huang

8. Co-Immunoprecipitation Assays to Detect In Vivo Association of Phytochromes with Their Interacting Partners

            Pengyu Song, Shaoman Zhang, and Jigang Li

9. Detection of UVR8 Homodimers and Monomers by Immunoblotting Analysis in Tomato

            Guoqian Yang, Xiaorui Liu, and Li Lin

10. Characterization of Seedling Greening Process in Plant Photomorphogenesis

            Wanqing Wang, Yuhong Li, and Rongcheng Lin

11. Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody

            Xiangguang Lyu, Hongyu Li, and Bin Liu

12. Uncover the Nuclear Proteomic Landscape with Enriched Nuclei Followed by Label-Free Quantitative Mass Spectrometry

            Yan Wang, Zhuang Lu, and Lei Wang

13. Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii

            Huanling Yang, Fei Han, Yue Wang, Wenqiang Yang, and Wenfeng Tu

14. Co-Immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants

            Jingyi Zhang and Shengbo He

15. Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay

            Xiaolong Hao and Ling Li

16. Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System

            Xu Li, Yawen Liu, and Hongtao Liu

17. Semi-In-Vivo Pull Down Assay for Blue Light-Dependent Protein Interactions

18. Tobacco System for Studying Protein Colocalization and Interactions

            Jingyi Zhang and Shengbo He