Jasmonate in Plant Biology: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
Part I Physiology and Development
1.Multiple Roles of Jasmonates in Shaping Rhizotaxis: Emerging IntegratorsAbdellah Lakehal, Alok Ranjan, and Catherine Bellini
2.Phenotyping of Light Response on JA Defective Mutant in RiceMichael Riemann 
3.Techniques to Determine Effects of Jasmonates on Root Hydraulic Conductivity Beatriz Sánchez-Romera and Ricardo Aroca 
Part II Biotic Interactions
4.The Role of Jasmonate in Beneficial Microbe-Root InteractionsVeronica Basso and Claire Veneault-Fourrey 
5.Plant-Insect Bioassay for Testing Arabidopsis Resistance to the Generalist Herbivore Spodoptera littoralisStefan Mielke and Debora Gasperini
6.Targeted Metabolomics of Plant Hormones and Redox Metabolites in Stomatal ImmunityLisa David, Jaining Kang, and Sixue Chen
7.Bioassays to Evaluate the Resistance of Whole Plants to the Herbivorous Insect Thrips Merel Steenbergen, Colette Broekgaarden, Corné M.J. Pieterse, and Saskia C.M. van Wees
8.Role of Jasmonate in Modulation of Mycorrhizae Induced Resistance against Fungal PathogensAswathy Nair, H.V. Thulasiram, and Sujata Bhargava 
9.Establishment of Actinorhizal Symbiosis in Response to Ethylene, Salicylic Acid, and JasmonateMariama Ngom, Maimouna Cissoko, Krystelle Gray, Valérie Hocher, Laurent Laplaze, Mame Ourèye Sy, Sergio Svistoonoff, and Antony Champion
Part III Metabolism and Signaling
10.Crystallization of a Complex between MYC and Jas MotifFeng Zhang, Sheng Yang, and Karsten Melcher 
11.A Fluorescence Anisotropy-Based Comprehensive Method for the In Vitro Screening of COI1-JAZs Agonists and AntagonistsYousuke Takaoka, Kengo Hayashi, Kaho Suzuki, Ika Nurul Azizah, and Minoru Ueda
12.Use of Fluorescent Reporters to Analyse Dynamic and Spatial Responses to Mechanical WoundingAntoine Larrieu, Trang Hieu Nguyen, and Antony Champion
13.Quantitative Jasmonate Profiling using a High-Throughput UPLC-nanoESI-MS/MS MethodCornelia Herrfurth and Ivo Feussner 
14.Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass SpectrometryLiu Yingyao, Rajesh N. Patkar, and Naweed I. Naqvi 
15.CRISPR/Cas9-Mediated Gene Editing of the Jasmonate Biosynthesis OsAOC Gene in RiceHieu Trang Nguyen, Huong To Thi Mai, Daniel Moukouanga, Michel Lebrun, Stephane Bellafiore, and Antony Champion
16.Mass Spectrometric Approaches to Study the Metabolism of Jasmonates: Biotransformation of Exogenously-Supplemented Methyl Jasmonate by Cell Suspension Cultures of Moringa oleiferaClaude Y. Hamany Djande, Ntakadzeni E. Madala, and Ian A. Dubery