Peptide and Protein Engineering: From Concepts to Biotechnological Applications

1. SEA Ligation is Accelerated at Midly Acidic pH: Application to the Formation of Difficult Peptide Junctions

            Marine Cargoët, Vincent Diemer, Laurent Raibaut, Elizabeth Lissy, Benoît Snella, Vangelis Agouridas, and Oleg Melnyk

2. The Problem of Aspartimide Formation during Protein Chemical Synthesis Using SEA-Mediated Ligation

            Jennifer Bouchenna, Magalie Sénéchal, Hervé Drobecq, Jérôme Vicogne, and Oleg Melnyk

            Vangelis Agouridas and Oleg Melnyk

4. Only a "Click" Away: Development of Arginine-Rich Peptide-Based Materials Using Click Chemistry

            Mariana Barbosa, Fabíola Costa, Cátia Teixeira, M. Cristina L. Martins, and Paula Gomes

5. Fluoroglycoproteins by Copper-Free Strain-Promoted Azide-Alkyne Cycloaddition

            Pedro M.S.D. Cal, Gonçalo J.L. Bernardes, and Omar Boutureira

6. Hybrid Silylated Peptides for the Design of Bio-Functionalized Materials

            Titouan Montheil, Cecile Echalier, Jean Martinez, Ahmad Mehdi, and Gilles Subra

7. Synthesis of Peptide-Oligoethylene Glycol (OEG) Conjugates for Multivalent Modification of Nanomaterials

            Daniel Pulido and Miriam Royo

8. Phage Display Methodologies

            Agathe Urvoas, Philippe Minard, and Patrice Soumillion

9. Whole-Bacterium Ribosome Display Selection for Isolation of Anti-Bacterial Affitins

            Ghislaine Béhar, Stanimir Kambarev, Jennifer Jazat, Barbara Mouratou, and Frédéric Pecorari

10. CIS Display: DNA-Based Technology as a Platform for Discovery of Therapeutic Biologics

            Ana Margarida Gonçalves Carvalho Dias

11. Building Scarless Gene Libraries in the Chromosome of Bacteria

            Gol Mohammad Dorrazehi, Sebastian Worms, Jason Baby Chirakadavil, Johann Mignolet, Pascal Hols, and Patrice Soumillion

12. Streptavidin (Sav)-Based Artificial Metalloenzymes: Cofactor Design Considerations and Large Scale Expression of Host Protein Variants

13. Integrated Bacterial Production of Large Combinatorial Libraries of Cyclic Oligopeptides and Functional Screening for the Identification of Chemical Rescuers of Pathogenic Protein Misfolding and Aggregation

            Dafni C. Delivoria and Georgios Skretas

14. Site-Specific Incorporation of Non-Canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli

            Uchralbayar Tugel, Meritxell Galindo, and Birgit Wiltschi