Caveolae: Methods and Protocols

Preface... Table of Contents... Contributing Authors...
1. Selective Visualization of Caveolae by TEM using APEX2 Alexander Ludwig
2. Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and Tissues Eric Seemann, Michael M. Kessels, and Britta Qualmann
3. Analysis of Caveolin in Primary Cilia Stine K. Morthorst, Johanne B. Mogensen, Søren T. Christensen, and Lotte B. Pedersen
4. Method for Efficient Observation of Caveolin-1 Plasma Membrane by Microscopy Imaging Analysis Tomoya Yamaguchi, Toyoshi Fujimoto, and Takashi Takahashi
5. Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the Plasma Membrane Takashi Hirama and Raibatak Das
6. Spatiotemporal Analysis of Caveolae Dynamics using Total Internal Reflection Fluorescence Microscopy Yosuke Senju and Shiro Suetsugu
7. Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch Adriana M. Zimnicka, Zhenlong Chen, Peter T. Toth, and Richard D. Minshall
8. GPMVs as a Tool to Study Caveolin-Interacting Partners Joanna Podkalicka and Cedric M. Blouin
9. Biotin Proximity Labeling to Identify Protein-Protein Interactions for Cavin1 Carolina Mendoza-Topaz
10. Investigation of Novel Cavin-1/Suppressor of Cytokine 3 (SOCS3) Interactions by Co-Immunoprecipitation, Peptide Pull-Down and Peptide Array Overlay Approaches Jamie J.L. Williams, George S. Baillie, and Timothy M. Palmer
11. Analysis of Protein and Lipid Interactions using Liposome Co-Sedimentation Assays Elin Larsson, Madlen Hubert, and Richard Lundmark
12. Liposome Binding Assay to Characterize Cavin Family Protein Structure and Functions Miriam Stoeber
13. Preparation of Caveolin-1 for NMR Spectroscopy Experiments Sarah M. Plucinsky, Jeffrey A. Julien, and Kerney J. Glover
14. Tagging and Deleting of Endogenous Caveolar Components using CRISPR/Cas9 Technology Elena Shvets and Carolina Mendoza-Topaz
15. Pulling of Tethers from the Cell Plasma Membrane using Optical Tweezers Darius V. Köster
16. Live Confocal Imaging of Zebrafish Notochord Cells under Mechanical Stress In Vivo Ye-Wheen Lim, Harriet P. Lo, Thomas E. Hall, and Robert G. Parton
17. Study of Caveolae Dependent Mechanoprotection in Huma Muscle Cells using Micropatterning and Live Cell Microscopy Melissa Dewulf and Cedric M. Blouin
18. Immunofluorescence-Based Analysis of Caveolin-3 in the Diagnostic Management of Neuromuscular Diseases Andreas Roos, Denisa Hathazi, and Ulrike Schara