Mouse Genetics: Methods and Protocols

1. Generation of Mouse Model (KI & CKO) via Easi-CRISPR

            Dorjee T.N. Shola, Chingwen Yang, Chiayun Han, Rada Norinsky, and Ruben D. Peraza

2. Analysis of Gene Expression Using lacZ Reporter Mouse Lines

            Michael Simon Krämer, Robert Feil, and Hannes Schmidt

3. Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice

            Benedetta Ruzzenente and Metodi D. Metodiev

4. Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology

            Fatih Semerci, Luke Parkitny, and Mirjana Maletic-Savatic

5. Retina as a Model to Study In Vivo Transmission of -Synuclein in the A53T Mouse Model of Parkinson's Disease

            Najiba Mammadova, Thierry Baron, Jérémy Verchère, Justin J. Greenlee, and M. Heather West Greenlee

6. Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas

            Mugdha V. Joglekar, Rohan R. Patil, Sarang N. Satoor, Wilson K.M. Wong, Mahesh S. Karandikar, and Anandwardhan A. Hardikar

7. Color-Coded Imaging of Cancer and Stromal Cells Interaction in the Pancreatic Cancer Tumor Microenvironment (TME)

            Atsushi Suetsugu and Robert M. Hoffman

8. Generating Ins2^+/-/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Diabetic Hearts in Humans

            Hamid R. Shahshahan, Tyler N. Kambis, Sumit Kar, Santosh K. Yadav, and Paras K. Mishra

9. Generating a Podocyte-Specific FcRn Knockout Mouse

            Judith Blaine

10. Mouse Models of Colitis-Associated Colon Cancer

            Santhakumar Manicassamy, Puttur D. Prasad, and Daniel Swafford

11. Colon Cancer Tumor Model Base on Colonoscopy Injection

            Suhwan Chang

12. Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse

            Jeffrey Barminko, Andrei M. Vacaru, and Margaret H. Baron

13. Gene Inactivation in Adult Long Term-Hematopoietic Stem Cells Using Inducible Mouse Models

            Kristbjorn Orri Gudmundsson

14. Hematological Humanization of Immune Deficient Mice

15. Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting

            Rika Maruyama and Toshifumi Yokota