Characterization of Plant Viruses: Methods and Protocols

Chapter 1. Glasshouse for maintenance of virus and insect culture.- Chapter 2. Symptoms of virus infected plants.- Chapter 3. Isolation and diagnosis of virus through indicator hosts.- Chapter 4. Host range of viruses.- Chapter 5. Physico-chemical properties of virus in crude sap.- Chapter 6. Mechanical sap transmission.- Chapter 7. Transmission through grafting and budding.- Chapter 8. Transmission through dodder.- Chapter 9. Virus transmission through Pollen.- Chapter 10. Transmission through seeds.- Chapter 11. Transmission of viruses by aphids.- Chapter 12. Transmission of viruses by leafhoppers.- Chapter 13. Transmission of virusesby whiteflies.- Chapter 14. Transmission of virusesby thrips.- Chapter 15. Transmission of viruses through mealybugs.- Chapter 16. Transmission of viruses through beetles.- Chapter 17. Transmission of viruses through mites.- Chapter 18. Transmission of viruses through fungi.- Chapter 19. Transmission of viruses through nematodes.- Chapter 20. Storage and preservation of plant virus cultures.- Chapter 21. Purification of Plant Viruses.- Chapter 22. Ultraviolet absorption spectra of purified virus preparation.- Chapter 23. Electron microscopy and Utramicrotomy.- Chapter 24. Determination of coat protein molecular weight of viruses.- Chapter 25. Isolation of nucleic acid from purified virus and determination of its nature.- Chapter 26. Agarose gel electrophoresis for nucleic acids.- chapter 27. In vitro expression of viral coat protein in prokaryotic system and its purification.- Chapter 28. Production of Polyclonal Antiserum.- Chapter 29. Production of monoclonal antibody.- chapter 30. Serological Tests.- Chapter 31. Isolation of total DNA from plants.- Chapter 32. Isolation of total RNA from plants.- Chapter 33. Isolation of double stranded (ds) RNA from virus infected plants.- Chapter 34. Dot-blot hybridization technique.- Chapter 35. Polymerase chain reaction.- Chapter 36. Real-time polymerase chain reaction.- Chapter 37. DNA Microarray for detection of plant viruses.- Chapter 38. Loop-mediated isothermal amplification (LAMP).- Chapter 39. Rolling circle amplification (RCA).- Chapter 40. Recombinase polymerase amplification.- Chapter 41. Next generation sequencing for diagnosis of viruses.- Chapter 42. Cloning of PCR Product.- Chapter 43. cDNA synthesis and cloning.- Chapter 44. DNA sequencing.- Chapter 45. Sequence analysis and phylogenetic studies.- Chapter 46. Development of infectious clone of virus.- Chapter 47. Virus elimination by meristem-tip culture.- Chapter 48. Virus elimination through somatic embryogenesis.- Chapter 49. Production of virus-resistant plants through transgenic approaches.- Chapter 50. Production of virus-resistant plants through CRISPR-Cas technology.