Cell Cycle Oscillators: Methods and Protocols

1. Cell Cycle Control: A System of Interlinking Oscillators

            Randy Y.C. Poon

2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity

            Matthew P. Swaffer

3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts

            Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr.

4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts

            Morgan Pellerano and May C. Morris

5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry

            Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall

6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots

7. Construction of a Full-Length 3'UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs

            Dominika Kazmierczak and Per Hydbring

8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis

            Mardo Kõivomägi

9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block

            Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte

10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition

            Pawan Singh, Lenka Halova, and Iain Michael Hagan

11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System

            Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur

12. EDU (5-Ethynyl-2'-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle

            João A. Ferreira, Marco Neves, Miguel Alpalhão, Pedro Pereira, Daniela Cunha, Fernando Ferreira, René Santus, Ana E. Sousa, and Paulo L. Filipe

13. High-Resolution Analysis of Centrosome Behavior During Mitosis

            Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira

14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells

            Jonathan M. Geisinger and Tim Stearns

15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle

16. Visualization of Radiation-Induced Cell Cycle Kinetics with a Fluorescent Ubiquitination-Based Cell Cycle Indicator (Fucci)

            Atsushi Kaida and Masahiko Miura

17. Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody

            Yuko Sato and Hiroshi Kimura

18. Analyzing Centrioles and Cilia by Expansion Microscopy

            Dong Kong and Jadranka Loncarek

19. Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae

            Deniz Pirincci Ercan and Frank Uhlmann

20. Application of PALM Super Resolution Microscopy to the Analysis of Microtubule-Organizing Centers (MTOCs) in Aspergillus nidulans

            Xiaolei Gao, Reinhard Fischer, and Norio Takeshita

21. Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools

            Melinda Van Kerckvoorde, Matthew J. Ford, Patricia L. Yeyati, Pleasantine Mill, and Richard L. Mort

22. Calorimetric Heat Dissipation Measurements of Developing Zebrafish Embryos

            Jonathan Rodenfels and Karla M. Neugebauer

23. The Conditional Knockout Analogous System: CRISPR-Mediated Knock Out Together with Inducible Degron and Transcription-Controlled Expression

            Hoi Tang Ma