Plant Mitochondria: Methods and Protocols

1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

            Huy Cuong Tran and Olivier Van Aken

2. Cell Type-Specific Isolation of Mitochondria in Arabidopsis

            Clément Boussardon and Olivier Keech

3. Isolation of Plant Mitochondria Using Affinity Purification

            Franziska Kuhnert and Andreas P.M. Weber

4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient

            Ian Max Møller and Allan G. Rasmusson

5. Integrity Assessment of Isolated Plant Mitochondria

            Allan G. Rasmusson, Mengshu Hao, and Ian Max Møller

6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores

            Brendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar

7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin

Allan G. Rasmusson, Ian Max Møller, and Susanne Widell

8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)

            Xuyen Le, A. Harvey Millar, and Chun Pong Lee

9. Complexome Profiling of Plant Mitochondrial Fractions

            Lucie Schröder, Holger Eubel, and Hans-Peter Braun

10. High Throughput BN-PAGE for Mitochondrial Respiratory Complexes

            Lilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small

11. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy

            Anqiang Tang, Yimin Wang, and Nicolas L. Taylor

12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana

            Cristina Ruberti

13. Assessment of Mitochondrial Protein Topology and Membrane Insertion

14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis

            Malgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska

15. Markers for Mitochondrial ROS Status

            Monika Ostaszewska-Bugajska, Anna Podgórska, and Bozena Szal

16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling

            Jonas Giese, Jürgen Eirich, Frederik Post, Markus Schwarzländer, and Iris Finkemeier

17. Analysis of the Plant Mitochondrial Transcriptome

            Kevin Baudry, Etienne Delannoy, and Catherine Colas des Francs-Small

18. Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing Data

            Mizuki Takenaka

19. Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana Mitochondria

            Omar Saleh, Sarlita Dwiani, Julia Rott, and Kristina Kühn

20. Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in Arabidopsis

            Déborah Schatz-Daas, Arnaud Fertet, Frédérique Lotfi, and José M. Gualberto

21. Measuring the Activity of DNA Repair Enzymes in Isolated Mitochondria

            Beatriz Ferrando, Ian Max Møller, and Tinna Stevnsner

22. MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes

            Shin-ichi Arimura