Cytoskeleton: Methods and Protocols

PART I                        IMAGING THE CYTOSKELETON

1          Super Resolution Imaging of the Actin Cytoskeleton in Living Cells Using TIRF-SIM

2          Imaging Cytoskeleton Components by Electron Microscopy

Tatyana Svitkina

Cierra N. Sing, Emily J. Yang, Theresa C. Swayne, Ryo Higuchi-Sanabria, Catherine A. Tsang, Istvan R. Boldogh, and Liza A. Pon

4          Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells

Cierra N. Sing, Emily J. Yang, Ryo Higuchi-Sanabria, Liza A. Pon, Istvan R. Boldogh, and Theresa C. Swayne

Gilberto Garcia, Stefan Homentcovschi, Naame Kelet, and Ryo Higuchi-Sanabria

6          Imaging the Cytoskeleton in Living Plant Roots

7          Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans

Shoichiro Ono

8          Imaging of the Cytoskeleton using Live and Fixed Drosophila Tissue Culture Cells

Derek A. Applewhite, Christine A. Lacy, Eric R. Griffis, and Omar A. Quintero

PART II                      CYTOSKELETON DYNAMICS

9          Influencing the Actin-Dynamics in Plant Cells by Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins

Andreas Holzinger

10        Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatworms

Labib Rouhana, Tracy Chong, and Phillip A. Newmark

11        An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells

            Luis Carlos Santos, Emilia Laura Munteanu, and Nicolas Biais

12        Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an ÄKTA Start System

Michael Gotesman, Yueyang Wang, Sharath Madasu, and Carter A. Mitchell

13        The Cilioprotist Cytoskeleton, a Model for Understanding How Cell Architecture and Pattern Are Specified: Recent Discoveries from Ciliates and Comparable Model Systems

            Linda A. Hufnagel

PART III                    CELL AND ORGANELLE MOTILITY

14        3D and 4D Tumorigenesis Model for the Quantitative Analysis of Cancer Cell Behavior and Screening for Anti-Cancer Drugs

Deborah Wessels, Daniel F. Lusche, Edward Voss, and David R. Soll

15        Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells

Christina King-Smith

16        Chemotaxis: Under Agarose Assay

Derrick Brazill and David A. Knecht

PART IV                     GENETIC AND PROTEOMIC PROTOCOLS            

17        Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila

Qi He and Christopher Roblodowski

18        Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila

19        Proteomic Tools for Analysis of Cytoskeleton Proteins

Carlos Barreto, Andriele Silva, Eliza Wiech , Antonio Lopez, Avdar San, and Shaneen Singh