Macromolecular Biorecognition: Principles and Methods

I. Specific Interactions in Proteins: Molecular Aspects and Functional Regulation.- Computer Graphics as an Aid in Protein Structure-Function Analysis and Design.- Computational Approaches to the Study of Protein-Ligand Interactions.- Synthetic Peptides and the Design of Peptide and Protein Recognition Surfaces.- Limited Proteolysis of Globular Proteins: Molecular Aspects Deduced from Studies on Thermolysin.- Crystallographic Studies on Retinol-Binding Protein and Beta-Lactoglobulin.- Structural Bases for the Recognition of Inhibitors by Serine Proteinases and Their Zymogens.- Role of the Primary Specificity Subsite on the Interaction Between Serine Proteinases and Low Molecular Weight Substrates and Inhibitors.- Interaction Between Serine (pro)Enzymes and Macro-molecular Inhibitors. Thermodynamic and Kinetic Aspects.- Subunit Interactions in Cytochrome Oxidase: The Role of Subunit III.- Myosin Subfragment 1 Catalyzes the Polymerization of Actin at Low Ionic Strength.- Structural Effects in the Self-Association of ?II-Crystallin.- The Role of Lysine-7 in Ribonuclease A.- II. Interaction of Nucleic Acids with Proteins and Drugs.- Transition of Chromatin from the "10 nm" Lower Order Structure to the "30 nm" Higher Order Structure, as Followed by Small Angle X-Ray Scattering.- Topology of DNA in its Interaction with Actinomycin D and with the Histone Octamer.- Thermodynamics and Stereochemistry of the Interaction Between Anthraquinone Drugs and DNA.- Interaction of cis-Ru(DMSO)4Cl2 with DNA in Aqueous Solution.- Studies on the Interaction Between Ribosome and Elongation Factor 2 by Fluorescent Labeling of the Diphthamide Residue.- III. Immunological Recognition and Development of Synthetic Vaccines.- Fine Specificity of Monoclonal Anti-Lysozyme Antibodies and the Three-Dimensional Structure of a Lysozyme-Antibody Complex.- Predicting Antigenicity in Proteins and Future of Synthetic Peptides.- Folding and Antigenicity of Thermolysin Fragments.- Synthesis and Biological Activity of the Immunomodulatory Site of Interleukin 1.- The 208-222 Region of the Peplomer Glycoprotein as a Putative Binding Site of Rabies Virus with the Nicotinic Acetylcholine Receptor.- Heterogeneity and Specificity of Murine Cell Response to Synthetic Peptide (NANP)n of P. falciparum Circumsporozoite Protein.- Mapping of Antigen Epitopes Interacting with Class II MHC Products and with the Antigen Receptor of T Lymphocytes.- IV. Analytical and Preparative Bioaffinity Methods.- Analytical Use of Antibodies Based on Specific Molecular Binding.- Modified Avidins in Detection Systems.- New Biospecific Chromatographies of ?-Hexosaminidases.- Preparative Applications of Subunit Exchange Chromatography.- Use of Monospecific Antibodies for the Purification of D-Amino Acid Oxidases From Various Sources.- Adsorption of Aromatic Molecules on Hydrophilic Gel Chromatography Media: Effect of Various Salts.- Author Index.