Flower Development: Methods and Protocols

Part I: Review and Overview Chapters

1. Flower Development in Arabidopsis - There is More to it than Learning your ABCs

Nathanael Prunet and Thomas Jack

2. Flower Development in the Asterid Lineage

Barry Causier and Brendan Davies

3. Grass Flower Development

Hiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba

4. Flower Diversity and Angiosperm Diversification

Pamela S. Soltis and Douglas E. Soltis

5. Flower Development - Open Questions and Future Directions

Frank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrándiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, José Luis Riechmann

PART II: Genetic and Phenotypic Analyses

6. Genetic Screens for Floral Mutants in Arabidopsis thaliana : Enhancers and Suppressors

Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen

7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development

Mona M. Monfared and Jennifer C. Fletcher

8. Genetic and Phenotypic Analyses of Petal Development in Arabidopsis

Judit Szécsi, Barbara Wippermann and Mohammed Bendahmane

9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice

Fang Chang, Zaibao Zhang, Yue Jin, and Hong Ma

10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana

Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma

11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis

Vicente Balanzà, Patricia Ballester, Monica Colombo, Chloé Fourquin, Irene Martínez-Fernández, and Cristina Ferrándiz

Part III: Microscopy and Histology

12. Microscopic Analysis of Arabidopsis Ovules

Balaji Enugutti and Kay Schneitz

13. Scanning Electron Microscopy Analysis of Floral Development

Robert G. Franks

14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue

Anna Medzihradszky, Kay Schneitz, and Jan U. Lohmann

15. The GUS Reporter System in Flower Development Studies

Janaki S. Mudunkothge and Beth A. Krizek

Part IV: Experimental Systems

16. A Floral Induction System for the Study of Early Arabidopsis Flower Development

Diarmuid Ó'Maoiléidigh and Frank Wellmer

17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types

G. Venugopala Reddy

18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers

Ying Wang and Yuling Jiao

19. Laser-assisted Microdissection Applied to Floral Tissues

Samuel E. Wuest and Ueli Grossniklaus

PartV: Molecular Biology, Genomics, and Systems Biology

20. Identification of Arabidopsis Knock-out Lines for Genes of Interest

José Tomás Matus, Thilia Ferrier, and José Luis Riechmann

21. Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues

Mariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and José Luis Riechmann

22. Misexpression Approaches for the Manipulation of Flower Development

Yifeng Xu, Eng-Seng Gan, and Toshiro Ito

23. Next Generation Sequencing Applied to Flower Development: RNA-Seq

Jun He and Yuling Jiao

24. Next-Generation Sequencing Applied to Flower Development: ChIP-Seq

Emmanuelle Graciet, Diarmuid Seosamh Ó'Maoiléidigh, and Frank Wellmer
25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler and Carolyn Ohno

26. Gene Regulatory Network Models for Floral Organ Determination

Eugenio Azpeitia, José Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla