Practical Protein Chromatography

Thiophilic Adsorption Chromatography. Immobilized Metal Ion Affinity Chromatography. Histidine Ligand Affinity Chromatography. Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes. Calcium-Dependent Hydrophobic Interaction Chromatography. Lectin Affinity Chromatography. Dye-Ligand Affinity Chromatography. High-Performance Affinity Chromatography for Protein Separation and Purification. Immunoaffinity Chromatography. Selection for Antibodies for Immunoaffinity Chromatography. Some Alternative Coupling Chemistries for Affinity Chromatography. Exploiting Weak Affinities. Biospecific Affinity Elution. Size-Exclusion High-Performance Liquid Chromatography of Proteins. Chromatofocusing. Ion-Exchange Chromatography of Proteins. Displacement Chromatography of Proteins. Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography. Making Bacterial Extracts Suitable for Chromatography. Determination of Purity and Yield. Index.