Peptide Libraries: Methods and Protocols

I. Chemical Strategies for Library Synthesis

1. Design, Synthesis and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries

Kit Lam and Ruiwu Liu

2. Synthesis of Macrocyclic Organo-Peptide Hybrids from Ribosomal Polypeptide Precursors via CuAAC-/Hydrazide-Mediated Cyclization

Jessica M. Smith and Rudi Fasan

3. Synthesis and Screening of One-Bead-One-Compound Cyclic Peptide Libraries

Ziqing Qian, Punit Upadhyaya, and Dehua Pei

4. Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation

Kristen A. Andersen and Ronald T. Raines

5. Macrocyclic Templates for Library Synthesis of Peptido-Conjugates

Serge Zaretsky, Joanne Tan, Jennifer L. Hickey, and Andrei K. Yudin

6. Synthesis and Alkylation of aza-Gly-Pro Building Blocks of Peptidomimetic Libraries for Developing Prostaglandin F2_ Receptor Modulators as Therapeutics to Inhibit Preterm Labour

Carine B. Bourguet and William D. Lubell

II. Chemistry and Genetically-Encoded Peptide Libraries

7. A Fragment Based Selection Approach for the Discovery of Peptide Macrocycles Targeting Protein Kinases

Elizabeth Restituyo, Karla Camacho-Soto, and Indraneel Ghosh

8. Peptide and Peptide Library Cyclization Via Bromomethyl Benzene Derivatives

David E. Hacker, Mohammed Almohaini, Aruna Anbazhagan, Zhong Ma, and Matthew C. T. Hartman

9. Phage Selection of Bicyclic Peptides Based on Two Disulfide Bridges

Shiyu Chen and Christian Heinis

10. De Novo Discovery of Bioactive Cyclic Peptides Using Bacterial Display and Flow Cytometry

Amol V. Shivange and Patrick S. Daugherty

11. Chemical Post-Translational Modification of Phage-Displayed Peptides

Simon Ng, Katrina F. Tjhung, Beth M. Paschal, Christopher J. Noren, and Ratmir Derda

12. Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries and Alanine-Scanning

Malgorzata E. Kokoszka and Brian K. Kay

13. Identifying Reactive Peptides from Phage-Displayed Libraries

Glenn M. Eldridge and Gregory A. Weiss

14. The Best and the Brightest: Exploiting Tryptophan-Sensitized Tb3+ Luminescence to Engineer Lanthanide-Binding Tags

Langdon J. Martin and Barbara Imperiali

III. Utility of Peptide Libraries

15. Synthesis and Cell-Based Screening of One-Bead One-Compound Peptide Libraries

F.C. Bononi and Len G. Luyt

16. Screening Peptide Array Library for the Identification of Cancer Cell Binding Peptides

Kamaljit Kaur, Sahar Ahmed, Rania Soudy, and Sarfuddin Azmi

17. Next Generation Sequencing of Phage-Displayed Peptide Libraries

Ratmir Derda and Wadim Matochko

18. Maleimide-Based Method for Elaboration of Cysteine-Containing Peptide Phage Libraries

Buyung Santoso and Brion W. Murray