Rab GTPases: Methods and Protocols

1. Rab Family of GTPases
Guangpu Li and M. Caleb Marlin

2. Bioinformatics Approaches to Identifying and Classifying Rab Proteins
Yoan Diekmann and Jose B. Pereira-Leal

3. Rab-NANOPS: FRET-Biosensors for Rab Membrane Nanoclustering and Prenylation Detection in Mammalian Cells
Arafath Kaja Najumudeen, Camilo Guzman, Itziar M. D. Posada, and Daniel Abankwa

4. High Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins
Ashwini K. Mishra and David G. Lambright

5. Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts
Ryan M. Nottingham and Suzanne R. Pfeffer

6. Analysis of the Interactions Between Rab GTPases and Class V Myosins
Andrew J. Lindsay, Stéphanie Miserey-Lenkei, and Bruno Goud

7. Assaying the Interaction of the Rab Guanine Nucleotide Exchange Factor Rabin 8 by Rab11
Danièle Stalder and Peter J. Novick

8. Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11
Shanshan Feng, Bin Wu, Johan Perånen, and Wei Guo

9. Ypt1 and TRAPP Interactions: Optimization of Multi-Color Bimolecular Fluorescence Complementation in Yeast
Zhanna Lipatova, Jane J. Kim, and Nava Segev

10. Identifying a Rab Effector on the Macro autophagy Pathway
Juan Wang, Serena Cervantes, Saralin Davis, and Susan Ferro-Novick

11. Functional Analysis of Rab27 and its Effector Slp-2a in Renal Epithelial Cells
Takao Yasuda, Paulina Mrozowska, and Mitsunori Fukuda

12. Small GTPases in Acrosomal Exocytosis
Matias A. Bustos, Ornella Lucchesi, Maria Celeste Ruete, Luis S. Mayorga, and Claudia N. Tomes

13. Rab Antibody Characterization: Comparison of Rab14 Antibodies
Andrew J. Lindsay and Mary W. McCaffrey

14. Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin
Yu Chen and Jennifer Lippincott-Schwartz

15. 3D Time-Lapse Analysis of Rab11/FIP5 Complex: Spatiotemporal Dynamics During Apical Lumen Formation
Anthony Mangan and Rytis Prekeris

16. In Vitro and In Vivo Characterization of the Rab11-GAP Activity of Drosophila Evi5
Carl Laflamme and Gregory Emery

17. Characterization of the Role Rab25 in Energy Metabolism and Cancer Using Extracellular Flux Analysis and Material Balance
Shreya Mitra, Jennifer Molina, Gordon B. Mills, and Jennifer B. Dennison

18. Measurement of Rab35 Activity by the GTP-Rab35 Trapper RBD35
Hotka Kobayashi, Kan Etoh, Soujiro Marubash, Norihiko Ohbayashi, and Mitsunori Fukuda

19. Analysis of Connecdenn 1-3 (DENN1A-C) GEF Activity for Rab35
Patrick D. Allaire, Peter S. McPherson, and Brigitte Ritter

20. Assay of Rab17 and its Guanine Nucleotide Exchange Factor Rabex-5 in Dendrites of Hippocampal Neurons
Yasunori Mori and Mitsunori Fukuda

21. Methods for Analysis of AP-3/Rabin4' in Regulation of Lysosome Distribution
Viorica Ivan and Peter van der Sluijs

22. Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay
Yaoyao Qi, Zhimin Liang, Zonghua Wang, Guodong Lu, and Guangpu Li

23. Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5
Rachel S. Salamon, Hashem A. Dbouk, Denise Collado, Jaclyn Lopiccolo, Anne R. Bresnick, and Jonathan M. Backer

24. Role of the Rab5 Guanine Nucleotide Exchange Factor, Rme-6, in the Regulation of Clathrin-Coated Vesicle Uncoating
Elizabeth Smythe
23. Identification of the Rab5 Binding Site in p110b - Assays for PI3Kb Binding to Rab5
Rachel S. Salamon, Hashem A. Dbouk, Denise Collado, Jaclyn Lopiccolo, Anne R. Bresnick, and Jonathan M. Backer

24. Role of the Rab5 Guanine Nucleotide Exchange Factor, Rme-6, in the Regulation of Clathrin-Coated Vesicle UncoatingElizabeth Smythe
25. Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells With or Without NGF
M. Caleb Marlin and Guangpu Li

26. Determining the Role of Rab7 in Constitutive and Ligand-Mediated Epidermal Growth Factor Receptor Endocytic Trafficking Using S