Plant Mitochondria: Methods and Protocols

1. Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa

Monika W. Murcha and James Whelan

2. Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5' End Maturation

Stefan Binder and Kristina Kühn

3. Mitochondrial Run-On Transcription Assay Using Biotin Labeling

Kristina Kühn

4. In Vitro RNA Uptake Studies in Plant Mitochondria

Szymon Kubiszewski-Jakubiak, Cyrille Megel, Elodie Ubrig, Thalia Salinas, Anne-Marie Duchêne, and Laurence Maréchal-Drouard

5. In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria

Owen Duncan, Chris Carrie, Yan Wang, and Monika W. Murcha

6. Plant Mitochondrial Proteomics

Nicolas L. Taylor and A. Harvey Millar

7. Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites

Markus Hartl, Ann-Christine König, and Iris Finkemeier

8. A Flowchart to Analyze Protease Activity in Plant Mitochondria

Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, and Elzbieta Glaser

9. Activity Measurements of Mitochondrial Enzymes in Native Gels

Peter Schertl and Hans-Peter Braun

10. Activity Assay for Plant Mitochondrial Enzymes

Shaobai Huang, Chun Pong Lee, and A. Harvey Millar

11. Analysis of Type II NAD(P)H Dehydrogenases

Kathleen L. Soole and Chevaun A. Smith

12. Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes

Richard P. Jacoby, A. Harvey Millar, and Nicolas L. Taylor

13. Micro-Respiration Measurements in Plants

Yun Shin Sew, A. Harvey Millar, and Elke Stroeher

14. Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation

Richard Fly, James Lloyd, Stephan Krueger, Alisdair Fernie, and Margaretha J. van der Merwe

15. Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana

Theresa J. Reape, Joanna Kacprzyk, Niall Brogan, Lee Sweetlove, and Paul F. McCabe

16. Imaging and Analysis of Mitochondrial Dynamics in Living Cells

Sanjaya B. Ekanayake, Amr M. El Zawily, Gaël Paszkiewicz, Aurélia Rolland, and David C. Logan

17. Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors

Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer, and Markus Schwarzländer

18. In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide

Marina Cvetkovska and Greg C. Vanlerberghe

19. Databases and Informatics Resources For Analysis of Plant Mitochondria

Reena Narsai

20. Expression and Crystallization of the Plant Alternative Oxidase

Benjamin May, Catherine Elliott, Momi Iwata, Luke Young, Julia Shearman, Mary S. Albury, and Anthony L. Moore