Chemotaxis: Methods and Protocols

1.      In Situ Assays of Chemotropism duringYeast Mating
DavidE. Stone and Robert A. Arkowitz

2.     ImagingPolarization in Budding Yeast
AllisonW. McClure, Chi-Fang Wu, Sam A. Johnson, and Daniel J. Lew

3.     Migration of Dictyostelium discoideum to theChemoattractant Folic Acid
KarlJ. Aufderheide and Chris Janetopoulos

4.     MitochondrialStress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideumCells
SuiLay, Oana Sanislav, Sarah J. Annesley, and Paul R. Fisher

5.     StudyingChemoattractant Signal Transduction Dynamics in Dictyostelium by BRET
A.F.M.Tariqul Islam, Branden M. Stepanski, and Pascale G. Charest

6.     Wave Patternsin Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals
GüntherGerisch and Mary Ecke

7.     ChemotacticBlebbing in Dictyostelium Cells
EvgenyZatulovskiy and Robert R. Kay

8.     DissectingSpatial and Temporal Sensing in DictyosteliumChemotaxis Using a Wave Gradient Generator
AkihikoNakajima and Satoshi Sawai

9.     Employing Dictyostelium as an Advantageous 3RsModel for Pharmacogenetic Research
GrantP. Otto, Marco Cocorocchio, Laura Munoz, Richard A. Tyson, Till Bretschneider,and Robin S.B. Williams

10.   Identificationof Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis
XiumeiCao and Jianshe Yan

11.   BiochemicalResponses to Chemically Distinct Chemoattractants during the Growth andDevelopment of Dictyostelium
NetraPal Meena and Alan R. Kimmel

12.   Live Imaging ofBorder Cell Migration in Drosophila
WeiDai and Denise J. Montell

13.   ShRNA-InducedGene Knock-Down In Vivo to Investigate Neutrophil Function
AbdulBasit, Wenwen Tang, and Dianqing Wu

14.   StudyingNeutrophil Migration In Vivo Using Adoptive Cell Transfer
YoshishigeMiyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster

15.   IntravitalTwo-Photon Imaging of Lymphocytes Crossing High Endothelial Venules andCortical Lymphatics in the Inguinal Lymph Node
ChungPark, Il-Young Hwang, and John H. Kehrl

16.   FlowCytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response
YuntaoWu

17.    VisualizingCancer Cell Chemotaxis and Invasion in 2D and 3D
OliviaSusanto, Andrew J. Muinonen-Martin, Max Nobis, and Robert H. Insall

18.    4D TumorigenesisModel for Quantitating Coalescence, Directed Cell Motility and Chemotaxis,Identifying Unique Cell Behaviors and Testing Anti-Cancer Drugs
SpencerKuhl, Edward Voss, Amanda Scherer, Daniel F. Lusche, Deborah Wessels, and DavidR. Soll

19.    An ExperimentalModel for Simultaneous Study of Migration of Cell Fragments, Single Cells andCell Sheets
Yao-HuiSun, Yuxin Sun, Kan Zhu, Bruce W. Draper, Alex Mogilner, and Min Zhao

21.    Visualization ofActin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy
MoritzWinterhoff, Stefan Brühmann, Christof Franke, Dennis Breitsprecher, and JanFaix

22.    QuantitativeMonitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal FluorescentMicroscopy
XuehuaXu, Michelle Yun, Xi Wen, Joseph Brzostowski, Wei Quan, Q. Jane Wang, and TianJin

23.    FluorescenceReadout of a Patch Clamped Membrane by Laser Scanning Microscopy
MatthiasGerhardt, Michael Walz, and Carsten Beta

24.    Use of ResonanceEnergy Transfer Techniques for In Vivo Detection of Chemokine ReceptorOligomerization
LauraMartínez-Muñoz, José Miguel Rodríguez-Frade, and Mario Mellado

25.    Multi-StateTransition Kinetics of Intracellular Signaling Molecules by Single-MoleculeImaging Analysis
SatomiMatsuoka, Yukihiro Miyanaga, and Masahiro Ueda

26.    Mathematics ofExperimentally Generated Chemoattractant Gradients
MartenPostma and Peter J.M. van Haastert