The Nuclear Envelope: Methods and Protocols
Lieferzeit: 7-14 Werktage
- Artikel-Nr.: 10374383
Beschreibung
Part I Nuclear Envelope Isolation
- Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome
Nadia Korfali, Laurence Florens, and Eric C. Schirmer
- Exploring the Protein Composition of the Plant Nuclear Envelope
Xiao Zhou, Kentaro Tamura, Katja Graumann, and Iris Meier
- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes
Samson Obado, Mark C. Field, Brian T. Chait, and Michael P. Rout
PartII Nuclear Envelope Protein Interactions, Localization, and Dynamics
4. Super Resolution Microscopy of the Nuclear Envelope and AssociatedProteins
Wei Xie, Henning F. Horn, and Graham D. Wright
- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS)
Shimi Takeshi, Chan-Gi Pack, and Robert D. Goldman
- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP
Krishna C. Mudumbi and Weidong Yang
The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro - Identifying Protein-Protein Associations at the Nuclear Envelope with BioID
Dae In Kim, Samuel C. Jensen, and Kyle J. Roux - In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners
Alice Barateau and Brigitte Buendia
Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components - Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation
Olga Tapia and Larry Gerace
- Analysis of Meiotic Telomere Behavior in the Mouse
Jana Link, Ricardo Benavente, and Manfred Alsheimer
Richard D. Byrne, Banafshé Larijani, and Dominic L. Poccia
Marek Drozdz and David J. Vaux
PartIII Nuclear Envelope Interactions with the Cytoskeleton
- Identification and Validation of Putative Nesprin Variants
Flavia Autore, Catherine M. Shanahan, and Qiuping Zhang
- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting
James Carthew and Iakowos Karakesisoglou
- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties
Rose-Marie Minaisah, Susan Cox, and Derek T. Warren
- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling
Gregory Fedorchak and Jan Lammerding
- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts
Wakam Chang, Susumu Antoku, and Gregg G. Gundersen
- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells
Meredith H. Wilson, Matthew G. Bray, and Erika L. F. Holzbaur - Imaging Approaches to Investigate Myonuclear Positioning in Drosophila
Mafalda Azavedo, Victoria K. Schulman, Eric Folker, Mridula Balakrishnana, and Mary Baylies
PartIV Nuclear Envelope-Chromatin Interactions
- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins
- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion Isabelle Dubant-Goulet
- DamID Analysis of Nuclear Organization in Caenorhabditis elegans
Georgina Gómez-Saldivar, Peter Meister, and Peter Askjaer
The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells - Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization
Craig S. Clements, Ural Bikkul, Mai Hassan Ahmed, Helen A. Foster, Lauren S. Godwin, and Joanna M. Bridger
- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System
Tobias Anton, Heinrich Leonhardt, and Yolanda Markaki
- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina
Suzanna Gonzalo and Ray Kreienkamp
Anja R. Oldenburg and Philippe Collas
Michael I. Robson and Eric C. Schirmer
PartV Nucleo-Cytoplasmic Transport
- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex
Martin W. Goldberg
- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane
Rosemarie Ungricht, Sumit Pawar, and Ulrike Kutay
- Nuclear Protein Transport in Digitonin Permeabilized Cells
Steven A. Adam
- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells
Ralph H. Kehlenbach and Sarah A. Port
- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport
Jiong Ma, Joseph M. Kelich, and Weidong Yang
Eigenschaften
Breite: | 186 |
Höhe: | 261 |
Länge: | 35 |
Seiten: | 523 |
Sprachen: | Englisch |
Autor: | Eric C. Schirmer, Philippe Collas, Sue Shackleton |