Plant Nitric Oxide: Methods and Protocols

1. An Overview ofMethods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use MultipleMethods?

            HideoYamasaki, Naoko S. Watanabe, Yasuko Sakihama, and Michael F. Cohen

2. Chemiluminescence Detection ofNitric Oxide from Roots, Leaves, and Root Mitochondria

            Aakanksha Wany, Alok K .Gupta, , Aprajita Kumari,  Shika Gupta,Sonal Mishra, Ritika Jaintu, Pradeep K. Phatak, and Kapuganti Jagadis Gupta

3. NitricOxide Measurement from Purified Enzymes and Estimation of Scavenging Activityby Gas Phase Chemlimunimescence Method

            Aprajita Kumari, Alok K.Gupta, Sonal Mishra, Aakanksha Wany, and Kapuganti Jagadis Gupta

4. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence

            Aakanksha Wany andKapuganti Jagadis Gupta

5. Nitric Oxide (NO) Measurements inStomatal Guard Cells

6. Methods to Detect Nitric Oxide in Plants: Are DAFsReally Measuring NO?

            Stefan Ruemer, MarkusKrischke, Agnes Fekete, Maria Lesch, Marin J. Mueller, and Werner M. Kaiser

7. ANovel Protocol for Detection of Nitric Oxide in Plants

            Prachi Jain and SatishC. Bhatla

8. Detection of Nitric Oxide by Electron ParamagneticResonance Spectroscopy: Spin-Trapping with Iron-Dithiocarbamates

            Luisa B. Maia and JoséJ.G. Moura

9. Measurement of Nitric Oxide (NO)Generation Rate by Chloroplasts Employing Electron Spin Resonance (ESR)

            Andrea Galatro and Susana Puntarulo

10. Laser-Based Methods for Detection of Nitric Oxide inPlants

11. Electrochemical Detection of Nitric Oxide in PlantCell Suspensions

            Sophie Griveau,Angélique Besson-Bard, Fethi Bedioui, and David Wendehenne

12. Quantification and Localization of S-Nitrosothiols (SNOs) in Higher Plants

            Juan B. Barroso, RaquelValderrama, Alfonso Carreras, Mounira Chaki, Juan C. Begara-Morales, BeatrizSánchez-Calvo, and Francisco J. Corpas

13. Analysis of the Expression and Activityof Nitric Oxide Synthase from Marine Photosynthetic Microorganisms

            Foresi Noelia, CorreaAragunde Natalia, Santolini Jerome, and Lamattina Lorenzo

14. Identificationof S-Nitrosothiols by the SequentialCysteine Blocking Technique

            Rafael A. Homem, ThierryLe Bihan, Manda Yu, and Gary J. Loake

15. Detectionof S-Nitrosoglutathione Reductase Activity in Plants

            Lucie Kubienová, TerezaTichá, Lenka Luhová, and Marek Petrivalský

16. Detectionof Peroxynitrite in Plants Exposed to Bacterial Infection

            Diana Bellin, MassimoDelledonne, and Elodie Vandelle