Heterologous Expression of Membrane Proteins: Methods and Protocols

1. Cell-Free Production of MembraneProteins in Escherichia coli Lysatesfor Functional and Structural Studies

            Ralf-Bernhardt Rues, Erik Henrich,Coilin Boland, Martin Caffrey, and Frank Bernhard

2. Membrane Protein Production in theYeast, S. cerevisiae

            Stephanie P. Cartwright, LinaMikaliunaite, and Roslyn M. Bill

3. Membrane Proteins Production in Escherichia coli: Protocols and Rules

            Federica Angius, Oana Ilioaia, Marc Uzan, and Bruno Miroux

4. Codon Optimizing for IncreasedMembrane Protein Production: A Minimalist Approach

            Kiavash Mirzadeh, Stephen Toddo,Morten H.H. Nørholm, and Daniel O. Daley

5. Generation of Tetracycline-InducibleMammalian Cell Lines by Flow Cytometry for Improved Overproduction of MembraneProteins

            Juni Andréll, Patricia C. Edwards,Fan Zhang, Maria Daly, and Christopher G. Tate

6. Membrane Protein Production in Lactococcus lactis for Functional Studies

            DaphneSeigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty,François André, Norbert Rolland, Edmund R.S. Kunji, and Annie Frelet-Barrand

7. Expression ofViral Envelope Glycoproteins in Drosophila melanogaster S2 Cells

            Renato Mancini Astray, Sandra Fernanda Suárez-Patiño, and Soraia AttieCalil Jorge

8. Leishmaniatarentolæ as a Promising Tool for Expressing Polytopic andMulti-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABCPump ABCG6

            Lucia Gonzalez-Lobato, VincentChaptal, Jennifer Molle, and Pierre Falson

9. Overexpression, Membrane Preparation,and Purification of a Typical Multidrug ABC Transporter BmrA

10. Expression ofEukaryotic Membrane Proteins in Pichiapastoris

16. Detergent-Free Membrane Protein Purification

            Alice J. Rothnie

17. Conformational Dynamics andInteractions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry

            Eric Forest and Petr Man

18. Lessons from an a-Helical Membrane Enzyme: Expression, Purification,and Detergent Optimization for Biophysical and Structural Characterization

            Jennifer L. Johnson, Sibel Kalyoncu,and Raquel L. Lieberman

19. Method to Screen Multidrug TransportInhibitors Using Yeast Over-Expressing a Human MDR Transporter

            Laura Fiorini and Isabelle Mus-Veteau