Mouse Genetics: Methods and Protocols

Part I: Mouse Genetics Techniques

1. Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre-loxP Site-Specific Recombination

Masato Ohtsuka

2. Generation of Conditional Knockout Mice

Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy and Kay-Uwe Wagner

3. Generating Mouse Lines for Lineage Tracing and Knockout Studies

Petra Kraus, V. Sivakamasundari, Xing Xing, and Thomas Lufkin

4. Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)

Anton J.M. Roebroek and Bart Van Gool

5. Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection

Kun-Hsiung Lee

6. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases

Susanne Feil, Jana Krauss, Martin Thunemann, and Robert Feil

7. Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice

Pedro N. Moreira and Lluís Montoliu

8. Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice

Jane Beil and Thorsten Buch

9. Generation of Genetically Engineered Mice by the piggyBac Transposon System

Sheng Ding, Tian Xu, and Xiaohui Wu

10. Generation and Applications of MADM-Based Mouse Genetic Mosaic System

Hui Zong

11. Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System

Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, and Vincenzo Coppola

12. In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis

Olga Simmons, Esther M. Bolanis, Jian Wang, and Simon J. Conway

Part II: Mouse Genetics in Stem Cells

13. Isolation and Handling of Mouse Embryonic Fibroblasts

Kanika Jain, Paul J. Verma, and Jun Liu

14. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue

Pollyanna Agnes Goh and Paul J. Verma

15. Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells

Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, and Mugdha V. Joglekar

16. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, and Margaret H. Baron

17. Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System

Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, and Yang Du

18. Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo

Lalit Sehgal, Abul Usmani, Sorab N. Dalal, and Subeer S. Majumdar

Part III: Mouse Genetics in Modeling Human Disease

19. Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts

Spencer W. Luebben, Naoko Shima, and Tsuyoshi Kawabata

20. Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment

Robert M. Hoffman

21. Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty

Viive M. Howell and Emily K. Colvin

22. Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans

Vishalakshi Chavali, Shyam Sundar Nandi, Shree Ram Singh, and Paras Kumar Mishra

23. Experimental Osteoarthritis Models in Mice

Julia Lorenz and Susanne Grässel

24. Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI)

Ganesan Ramesh and Punithavathi Ranganathan

25. Mouse Models of Acute and Chronic Colitis

Santhakumar Manicassamy and Indumathi Manoharan

26. Manipulation and Assessment of Gut Microbiome for Metabolic Studies

Sarang N. Satoor, Deepak P. Patil, Holly Kristensen, Mugdha V. Joglekar, Yogesh Shouche, and Anandwardhan A. Hardikar