Hematopoietic Stem Cell Protocols

Part I. Overview

1. The Hematopoietic Stem Cell Landscape

Kevin D. Bunting and Cheng-Kui Qu

Part II. Stem Cell Enrichment and Heterogeneity

2. Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow

Huimin Cao, Brenda Williams, and Susan K. Nilsson

3. Single-Cell PCR Profiling of Gene Expression in Hematopoiesis

Jose Teles, Tariq Enver, and Cristina Pina

4. Hematopoietic Stem and Progenitor Cell Mobilization in Mice

Jonathan Hoggatt, Tiffany A. Tate, and Louis M. Pelus

5. Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells

Brahmananda Reddy Chitteti and Edward F. Srour

6. Flow Cytometry Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells

Lei Dong and Cheng-Kui Qu

Part III. Molecular and Cellular Characterization

7. Gene Expression Profiling of Hematopoietic Stem Cells (HSC)

Nalini Raghavachari

8. Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells

Wenhuo Hu and Christopher Y. Park

9. DNA Methylation Profiling of Hematopoietic Stem Cells

Amber H . Begtrup

10. Metabolic Characterization of Hematopoietic Stem Cells

Fatih Kocabas, Junke Zheng, Chengcheng Zhang, and Hesham A. Sadek

11. Nano Proteomic Assays on Hematopoietic Stem Cells

Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, and Kevin D. Bunting

Part IV. In vitro Assays and Differentiation

12. Hematopoietic Differentiation of Pluripotent Stem Cells in Culture

Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, and Deborah L. French

13. Ex vivo Assays to Study Self-Renewal, Long-Term Expansion and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells

Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, and Jan Jacob Schuringa

14. Ex vivo Expansion of Murine and Human Hematopoietic Stem Cells

Phuong L. Doan and John P. Chute

15. Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level

Byungwook Aha, Zhengqi Wang, David R. Archer, and Wilbur A. Lam

Part V. Transplantation Assays and Imaging Engraftment

16. Five Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells

Ryo Yamamoto, Yohei Morita, and Hiromitsu Nakauchi

17. Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull

Juwell W. Wu, Judith M. Runnels, and Charles P. Lin

18. Immune Deficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development

Ken-Edwin Aryee, Leonard D. Shultz, and Michael A. Brehm

19. Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells

Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, and Linheng Li

Part VI. Genetic Modification

20. Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells

Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, and David A. Williams

21. Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells

Niek P. van Til and Gerard Wagemaker

22. High Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies

Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, and Hans-Peter Kiem

23. Barcoded Vector Libraries and Retro- or Lentiviral Barcoding of Hematopoietic Stem Cells

Leonid V. Bystrykh, Gerald de Haan, and Evgenia Verovskaya