Lentivirus Gene Engineering Protocols

Part I: Overview 1. Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions Jean-Luc Dreyer Part II: Advances in Lentiviral Vector Technology 2. New Protocol for Lentiviral Vector Mass Production María Mercedes Segura, Alain Garnier, Yves Durocher, Sven Ansorge, and Amine Kamen 3. Flexibility in Cell Targeting by Pseudotyping Lentiviral Vectors Daniela Bischof and Kenneth Cornetta 4. Doxycycline Regulated Lentiviral Vectors David Markusic and Jurgen Seppen 5. Strategies to Insulate Lentiviral Vector-Expressed Transgenes Ali Ramezani and Robert G. Hawley 6. Integrase Defective, Nonintegrating Lentiviral Vectors Zuleika Michelini, Donatella Negri, and Andrea Cara 7. Lentivirus-Based Virus-Like Particles as a New Protein Delivery Tool Claudia Muratori, Roberta Bona, and Maurizio Federico Part III: New Lentiviral Vector Applications 8. Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells Zhong-Wei Du and Su-Chun Zhang 9. Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells Mark S.K. Chong and Jerry Chan 10. Manipulating the Cell Differentiation Through Lentiviral Vectors Valeria Coppola, Cesare Galli, Maria Musumeci, and Désirée Bonci 11. Lentiviral Vector Transduction of Dendritic Cells for Novel Vaccine Strategies Lung-Ji Chang Part IV: The Short RNA Technologies Applied by Lentiviral Vectors 12. Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs Michaela Scherr, Letizia Venturini, and Matthias Eder 13. The RNA Silencing Technology Applied by Lentiviral Vectors inOncology Hidetoshi Sumimoto and Yutaka Kawakami 14. Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy Olivier ter Brake, Jan-Tinus Westerink, and Ben Berkhout 15. Lentivirus-Expressed siRNA Vectors Against Alzheimer Disease Kevin A. Peng and Eliezer Masliah