Plant Kinases: Methods and Protocols

1. Guide to the Book Plant Kinases

Nico Dissmeyer and Arp Schnittger

2. The Age of Protein Kinases

Nico Dissmeyer and Arp Schnittger

3. Expression and Purification of Active Protein Kinases from Wheat Germ Extracts

Boglárka Sonkoly, Viola Bardóczy, and Tamás Mészáros

4. Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System

Hirofumi Harashima and Masami Sekine

5. Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants

Róbert Dóczi, Elizabeth Hatzimasoura, and László Bögre

6. Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks

Nico Dissmeyer and Arp Schnittger

7. Bacterial Assay to Study Plant Sensor Histidine Kinases

Lukás Spíchal

8. Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase

Julija Umbrasaite, Alois Schweighofer and Irute Meskiene

9. Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen

Shutian Li and Vernonica E. Franklin-Tong

10. Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules

Dor Salomon, Chao Zhang, Kevan M. Shokat, and Guido Sessa

11. Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization

Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser

12. Quantifying Degradation Rates of Trans-Membrane Receptor Kinases

Niko Geldner

13. Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta

Mark A.C.J. Kwaaitaal, Marieke Schor, Mark A. Hink, Antonie J.W.G. Visser, and Sacco C. de Vries

14. Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo

Stefan Pusch, Nico Dissmeyer, and Arp Schnittger

15. Chemical Genetic Analysis of Protein Kinase Function in Plants

Mike Böhmer, Michael Bölker, and Tina Romeis

16. Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes

Thomas Colby, Horst Röhrig, Anne Harzen, and Jürgen Schmidt

17. Phosphoproteomics Using iTRAQ

Alexandra M.E. Jones and Thomas S. Nühse