Plant Organogenesis: Methods and Protocols

1. The Tomato Leaf as a Model System for Organogenesis

Yogev Burko and Naomi Ori

2. Usefulness of Physcomitrella patens for Studying Plant Organogenesis

Sandrine Bonhomme, Fabien Nogué, Catherine Rameau, and Didier G. Schaefer

3. The Dicot Root as a Model System for Studying Organogenesis

Julie Lavenus, Michael Lucas, Laurent Laplaze, and Soazig Guyomarc'h

4. Genetic Control of Root Organogenesis in Cereals

Caroline Marcon, Anja Paschold, and Frank Hochholdinger

5. Gene Expression Analysis of Aquatic Angiosperms Podostemaceae to Gain Insight into the Evolution of Their Enigmatic Morphology

Satoshi Koi and Natsu Katayama

6. Brown Algae as a Model for Plant Organogenesis

Kenny A. Bogaert, Alok Arun, Susana M. Coelho, and Olivier De Clerck

7. Microscopic Analysis of Ovule Development in Arabidopsis thaliana

Balaji Enugutti, Maxi Oelschner, and Kay Schneitz

8. Imaging of Phenotypes, Gene Expression and Protein Localization During Embryonic Root Formation in Arabidopsis

Cristina Llavata-Peris, Annemarie Lokerse, Barbara Möller, Bert De Rybel, and Dolf Weijers

9. Inducible System for Lateral Roots in Arabidopsis thaliana and Maize

Leen Jansen, Boris Parizot, and Tom Beeckman

10. Adventitious Root Induction in Arabidopsis thaliana as a Model for in vitro Root Organogenesis

Inge Verstraeten, Tom Beeckman, and Danny Geelen

11. High-Throughput, Non-Invasive Imaging of Root Systems

Anjali S. Iyer-Pascuzzi, Paul R. Zurek, and Philip N. Benfey

12. Small Molecule Screens to Study Lateral Root Development

Dominique Audenaert, Bert De Rybel, Long Nguyen, and Tom Beeckman

13. Cell Lineage Analyses in Living Tissues

John Runions and Smita Kurup

14. Protein Immunolocalization in Maize Tissues

Cristian Forestan, Nicola Carraro, and Serena Varotto

15. Auxin Immunolocalization in Plant Tissues

Cristian Forestan and Serena Varotto

16. Gene Expression Analysis of Shoot Apical Meristem Cell Types

Ram Yishor Yadav, Stephen Snipes, Thomas Girke, and G. Venugopala Reddy

17. Kinematic Analysis of Cell Division in Leaves of Mono- and Dicotyledonous Species: A Basis for Understanding Growth and Developing Refined Molecular Sampling Strategies

Hilde Nelissen, Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabuo Fiorani, and Gerrit T.S. Beemster

18. Regeneration in Arabidopsis Tissue Culture

Kaoru Sugimoto and Elliot M. Meyerowitz

19. Isolation and Analysis of mRNAs from Specific Cell Types of Plants by Ribosome Immunopurification

Angelika Mustroph, Eugenia Zanetti, Thomas Girke, and Julia Bailey-Serres

20. Analyzing Small and Long RNAs in Plant Development Using Non-Radioactive in-situ Hybridization

Pilar Bustos-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi

21. Analyzing Protein Distribution in Plant Tissues Using "Whole-Mount" Immunolocalization

Pilar Bustons-Sanmamed, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi

22. Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus

Aude Le Bail and Bénédicte Charrier

23. Building Simulation Models of Developing Plant Organs Using VirtualLeaf

Roeland M.H. Merks and Michael Guravage