Necrosis: Methods and Protocols

Part I: Analysis of Necrosis In Vitro

1. Navigation to the Graveyard- Induction of Various Pathways of Necrosis and their Classification by Flow Cytometry

Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens, Kirsten Lauber, Martin Herrmann

2. Time-lapse Imaging of Necrosis

Fredrik Wållberg, Tencho Tenev, and Pascal Meier

3. Activity Assays for Receptor Interacting Protein Kinase 1: A Key Regulator of Necroptosis

Jenny L. Maki and Alexei Degterev

4. Fluorescent Biosensors for the Detection of HMGB1 Release

Isabelle Martins, Oliver Kepp, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Erika Vacchelli, Lorenzo Galluzzi , and Guido Kroemer

Part II: Alternative Cell Death Modalities

5. Methods for the Study of Entosis

Qiang Sun and Michael Overholtzer

6. Analysis of Pyroptosis in Bacterial Infection

Lia Danelishvili and Luiz E Bermudez

Part III: Analysis of Cell Death in Mammalian Tissues

7. Analysis of Cell Death by Electron Microscopy

S. Burattini and E. Falcieri

8. Histological Analysis of Neurodegeneration in the Mouse Brain

Hiroo Yamaguchi and Jie Shen

9. Quantitation of Acute Necrosis after Experimental Myocardial Infarction

Xin-Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, & Edward B. Thorp

10. Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin

Marion C. Bonnet

Part IV: Cell Death in Non-Mammalian Model Systems

11. Identification and Characterization of Programmed Cell Death Markers in Bacterial Models

Daniel J. Dwyer and Jonathan A. Winkler

12. Quantification of Genetically Controlled Cell Death in Budding Yeast

Xinchen Teng and J. Marie Hardwick

13. Necrosis in C. elegans

Matt Crook, Avni Upadhyay and Wendy Hanna-Rose

14. Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans

Zao Li, Nan Lu, Xiangwei He, and Zheng Zhou

15. Modeling Calcium-overload Mediated Necrosis in Drosophila

Kai Liu, Yuhong Li and Lei Liu

16. Use of Necrotic Markers in the Drosophila Ovary

Allison K. Timmons, Tracy L. Meehan, Tori D. Gartmond, and Kimberly McCall

17. Detection and Measurement of Necrosis in Plants

Elena A. Minina, Lada H. Filonova, Geoffrey Daniel, and Peter V. Bozhkov