Tissue Microarrays: Methods and Protocols

Tissue Microarrays: Methods and ProtocolsEditor Ronald SimonTable of Contents:Table of Contents PrefaceContributors1. Applications of tissue microarray technologyRonald Simon2. Quality aspects of TMA analysis Pierre Tennstedt and Guido Sauter3.Representativity of TMA studies Guido Sauter4.Recipient block TMA techniqueMartina Mirlacher and Ronald Simon5. Protocol for constructing tissue arrays by cutting edge matrix assembly Thai Hong Tran, Justin Lin, Ashley Brooke Sjolund, Fransiscus Eri Utama, and Hallgeir Rui 6. Hypodermic needle without recipient paraffin block techniqueAndréa Rodrigues Cordovil Pires and Simone Rabello de Souza7. Resin Technologies: Constructing and staining of resin TMAsWilliam J Howat and Susan J Wilson8. Tissue microarrays from frozen tissues - OCT techniqueMarlena Schoenberg Fejzo and Dennis J. Slamon9.An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue SamplesZhongting Hu, Elbert Chang, Melissa Hodeib, and Joseph D. Abad 10.Building "Tissue" Microarrays from Suspension Cells Shuchun Zhao and Yasodha Natkunam 11. TMAs from Biopsy specimensMilton W. Datta and André A. Kajdacsy-Balla, 12.Immunohistochemical analysis of tissue microarraysRonald Simon, Martina Mirlacher and Guido Sauter13.DNA copy number analysis on Tissue MicroarraysAnne Kallioniemi14. RNA expression analysis on formalin-fixed paraffin-embedded (FFPE) tissue in TMA format by RNA in situ hybridization Jürgen Veeck and Edgar Dahl15.Automated analysis of Tissue MicroarraysMarisa Dolled-Filhart, Mark Gustavson, Robert L. Camp, David L. Rimm, John L. Tonkinson, and Jason Christiansen16.Digital microscopy for boosting database integration and analysis in TMA studies Tibor Krenacs, L. Ficsor, V. S. Varga , V. Angeli , and B. Molnar17.From Gene to Clinic: TMA based clinical validation of molecular markers in prostate cancerT. Schlomm, F. K. Chun, and A.Erbersdobler