Cell Cycle Synchronization: Methods and Protocols

1. Overview of Cell Synchronization

Gaspar Banfalvi

2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

Gaspar Banfalvi

3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer

4. Large Scale Mitotic Cell Synchronization

Kalyan Dulla and Anna Santamaria

5. Synchronization of Mammalian Cell Cultures by Serum Deprivation

Thomas J. Langan and Richard C. Chou

6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry

Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka

7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes

Takashi Iwamatsu

8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis

Congjun Li

9. Analysis of Nuclear Uracil DNA-Glycosylase (nUDG) Turnover during the Cell Cycle

Jennifer A. Fischer and Salvatore Caradonna

10. Synchronization of HeLa Cells

Hoi Tang Ma and Randy Y.C. Poon

11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

Gaspar Banfalvi

12. Synchronization of Yeast

Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider

13. Synchronization of Pathogenic Protozoans

Staffan Svärd and Karin Troell

14. Synchronization of In Vitro Maturation in Porcine Oocytes

Tamas Somfai and Yuji Hirao

15. Synchronization of Medicago sativa Cell Suspension Culture

Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth

16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)

Yoel Shufaro and Benjamin E. Reubinoff

17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

Kap-Hyoun Ko, Robert Nordon, Tracey A. O'Brien, Geoff Symonds, and Alla Dolnikov

18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients

Fabienne Venet, Caroline Guignant, and Guillaume Monneret

19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish

Attila Csikász-Nagy, Alida Palmisano, and Judit Zámborszky