Transgenic Cotton: Methods and Protocols

Part I. Introduction

1. Transgenic Cotton: From Biotransformation Methods to Agricultural Application

Baohong Zhang

2. Genetically Modified Cotton in India and Detection Strategies

Gurinder Jit Randhawa and Rashmi Chhabra

Part II. Transformation

3. Agrobacterium-Mediated Transformation of Cotton

Baohong Zhan

4. Biolistic Transformation of Cotton Zygotic Embryo Meristem

Rajah Rajasekaran

5. Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures

Rajah Rajasekaran

6. Cotton Transformation via Pollen Tube Pathway

Baohong Zhang and Qinglian Wang

7. Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.)

Muhammad Arshad, Yusuf Zafar, and Shaheen Asad

Part III. Detection

8. Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome

Jun Zhang and Yan Hong

9. Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR

Chengxin Yi and Yan Hong

10. Development of Enzyme-Linked Immunosorbant (ELISA) Assay for the Detection of Bt Protein in Transgenic Cotton

Abhishek Ojha, Arvind Kumar, and Suchitra Kamle

11. DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events

Gurinder Jit Randhawa, Monika Singh, and Rashmi Chhabra

12. A Simple and Rapid Method for Determining Transgenic Cotton Plants

Baohong Zhang, Hongmei Wang, Fang Liu, and Qinglian Wang

13. An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants

Min Wang, Qinglian Wang, and Baohong Zhang

14. Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoerpa armigera in Upland Cotton

Baolong Zhang, Wangzhen Guo, and Tianzhen Zhang

Part IV. Application

15. Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics

Hee Jin Kim

16. Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation

Baohong Zhang, Min Wang, Xin Zhang, Chengqi Li, and Qinglian Wang

17. Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover

Zhi Liu, Zhen Zhu, and Tianzhen Zhang

18. Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition

Zhiying Ma, Jianfeng Liu, and Xingfen Wang

19. Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb

Weiguo Miao and Jingsheng Wang

20. Development of Insect-Resistant Transgenic Cotton with Chimeric TVip3A Accumulating in Chloroplasts

Jiahe Wu and Yingchuan Tian

Part V. Risk Assessment

21. Determining Gene Flow in Transgenic Cotton

Xiaoping Pan