Single-Stranded DNA Binding Proteins: Methods and Protocols

1. Functions of Single-Strand DNA-Binding Proteins in DNA Replication, Recombination and Repair

Aimee H. Marceau

2. Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs

S. M. Arif and M. Vijayan

3. SSB Binding to ssDNA Using Isothermal Titration Calorimetry

Alexander G. Kozlov and Timothy M. Lohman

4. SSB-DNA Binding Monitored by Fluorescence Intensity and Anisotropy

Alexander G. Kozlov, Roberto Galletto, and Timothy M. Lohman

5. Single Molecule Analysis of SSB Dynamics on Single Stranded DNA

Ruobo Zhou and Taekjip Ha

6. Sample Preparation Methods to Analyze DNA-induced Structural Changes in Replication Protein A (RPA)

Chris A. Brosey, Susan E. Tsutakawa, and Walter J. Chazin

7. Structural Studies of SSB Interaction with RecO

Mikhail Ryzhikov and Sergey Korolev

8. Investigation of Protein-Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation

Natalie Naue and Ute Curth

9. Ammonium Sulfate Co-Precipitation of SSB and Interacting Proteins

Aimee Marceau

10. Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy

Duo Lu

11. Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins

Brian W. Walsh, Justin S. Lenhart, Jeremy W. Schroeder, and Lyle A. Simmons

12. Methods for Analysis of SSB/Protein Interactions by SPR

Asher N. Page and Nicholas P. George

13. Use of Native Gels to Measure Protein Binding to SSB

Jin Inoue and Tsutomu Mikawa

14. Identification of Small Molecules That Disrupt SSB/Protein Interactions Using a High-Throughput Screen

Douglas A. Bernstein

15. Detection of Post-translational Modifications of Replication Protein A

Cathy S. Hass, Ran Chen, and Marc S. Wold

16. Detecting Posttranslational Modification of Bacterial SSB Proteins

Dusica Vujaklija and Boris Macek

17. Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA

Katy Hedgethorne and Martin R. Webb

18. Fluorescent Single-Stranded DNA-Binding Proteins Enable in vitro and in vivo Studies

Piero R. Bianco, Adam J. Stanenas, Juan Liu, and Christopher S. Cohan

19. Use of Fluorescently Tagged SSB Proteins in in vivo Localization Experiments

Rodrigo Reyes-Lamothe

Douglas A. Bernstein

15. Detection of Post-translational Modifications of Replication Protein A

Cathy S. Hass, Ran Chen, and Marc S. Wold

16. Detecting Posttranslational Modification of Bacterial SSB Proteins

Dusica Vujaklija and Boris Macek

17. Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA

Katy Hedgethorne and Martin R. Webb

18. Fluorescent Single-Stranded DNA-Binding Proteins Enable in vitro and in vivo Studies

Piero R. Bianco, Adam J. Stanenas, Juan Liu, and Christopher S. Cohan

19. Use of Fluorescently Tagged SSB Proteins in in vivo Localization Experiments

Rodrigo Reyes-Lamothe