Campylobacter jejuni: Methods and Protocols

Campylobacter jejuni Isolation/Enumeration from Environmental Samples.- Recovery of Campylobacter spp. from Food and Environmental Sources.- Methods for Isolation, Purification, and Propagation of Bacteriophages of Campylobacter jejuni.- Drug Resistance Detection in Campylobacter.- Method of Peptide Nucleic Acid (PNA)-Mediated Antisense Inhibition of Gene Expression in Campylobacter jejuni.- Identification of Ligand-receptor Interactions: Ligand Molecular Arrays, SPR and NMR Methodologies.- Characterization of High Affinity Iron Acquisition Systems in Campylobacter jejuni.- Method for the Successful Crystallization of the Ferric Uptake Regulator from Campylobacter jejuni.- Methods for Initial Characterization of Campylobacter jejuni Bacteriophages.- Methods to Assess the Direct Interaction of C. jejuni with Mucins.- Methods to Study Campylobacter jejuni Adherence to and Invasion of Host Epithelial Cells.- Assays to Study the Interaction of Campylobacter jejuni with the Mucosal Surface.- Characterisation of Ligand-receptor Interactions: Chemotaxis, Biofilm, Cell Culture Assays, and Animal Model Methodologies.- Using Galleria Mellonella as an Infection Model for Campylobacter jejuni Pathogenesis.- Mouse Models for Campylobacter jejuni Colonization and Infection.- Metabolomic Analysis of Campylobacter jejuni by Direct Injection Electrospray Ionisation Mass Spectrometry.- Methods for Genome-wide Methylome Profiling of Campylobacter jejuni.- Characterizing Glycoproteins by Mass Spectrometry in Campylobacter jejuni.- Methods for Proteome Analysis of Campylobacter jejuni using 2-D Electrophoresis.- Analyzing Prokaryotic RNA-seq Data: A Case Study Identifying Holo-Fur Regulated Genes in Campylobacter jejuni.- Generation and Screening of an Insertion Sequencing-compatible Mutant Library of Campylobacter jejuni.