Brassinosteroids: Methods and Protocols

1. Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues

            Danuse Tarkowská and Miroslav Strnad

2. Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye

            Johan M. Winne, Niloufer G. Irani, Jos Van den Begin, and Annemieke Madder

3. Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice

            Hongning Tong and Chengcai Chu

4. Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness

            Claudia Corvalán and Sunghwa Choe

5. Approaches to Study Light Effects on Brassinosteroid Sensitivity

            Sandi Paulisic, Maria José Molina-Contreras, Irma Roig-Villanova, and Jaime F. Martínez-García

6. A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity

            Rosa Lozano-Durán and Youssef Belkhadir

7. Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing

            Trevor Nolan, Sanzhen Liu, Hongqing Guo, Lei Li, Patrick Schnable, and Yanhai Yin

8. Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA

            Kristina Vragovic, Elizabeth Bartom, and Sigal Savaldi-Goldstein

9. Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development

10. Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells

            Yu Luo and Eugenia Russinova

11. Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay

            Simon J. Unterholzner, Wilfried Rozhon, and Brigitte Poppenberger

12. Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry

            Walter van Dongen, Luc van Heerde, Sjef Boeren, and Sacco C. de Vries

13. Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment

            Carina A. Collins, Michelle E. Leslie, Scott C. Peck, and Antje Heese

14. Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation

15. The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops

            David Blasco, Fidel Lozano-Elena, Norma Fàbregas, and Ana I. Caño-Delgado

16. Brassinosteroid Action in Plant Abiotic Stress Tolerance

            Priti Krishna, Bishun D. Prasad, and Tawhidur Rahman