Protein Terminal Profiling: Methods and Protocols

Preface...
Table of Contents...
Contributing Authors...

1. [ 14 C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay
Adrian Drazic and Thomas Arnesen

2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
Håvard Foyn, Paul R. Thompson, and Thomas Arnesen

3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation
Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel

4. Profiling of Protein N-Termini and their Modifications in Complex Samples
Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen

5. Protease Substrate Profiling by N-Terminal COFRADIC
An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens

6. Doublet N-Terminal Oriented Proteomics for N-Terminomics and Proteolytic Processing Identification
Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss

7. Multidimensional Analysis of Protease Substrates and their Cellular Origins in Mixed Secretomes from Multiple Cell Types
Pascal Schlage and Ulrich auf dem Keller

8. System-Wide Profiling of Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissue Specimens for the Identification of Novel Substrates
Zon W. Lai and Oliver Schilling

9. Identification of Carboxypeptidase Substrates by C-Terminal COFRADIC
Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme

10. ProC-TEL: Profiling of Protein C-Termini by Enzymatic Labeling
Wenwen Duan and Guoqiang Xu

11. Determining Protease Substrates within a Complex Protein Background using the PROtein TOpography and Migration Analysis Platform (PROTOMAP)
R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, J.A. Clements

12. Multiplexed Protease Specificity Profiling Using Isobaric Labeling
Joanna Tucher and Andreas Tholey

13. FPPS - Fast Profiling of Protease Specificity
Matej Vizovisek, Robert Vidmar, and Marko Fonovic

14. Profiling of Protease Cleavage Sites by Proteome-Derived Peptide Libraries and Quantitative Proteomics
Chia-yi Chen, Bettina Mayer, and Oliver Schilling

15. Prediction of Proteases Involved in Peptide Generation
Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein

16. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches
Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane

17. Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors
Angelina Hahlbrock, Dorothée Gößwein, and Roland H. Stauber

18. Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method
Catharina Conrad, Miles A. Miller, Jörg W Bartsch, Uwe Schlomann, Douglas A. Lauffenburger

19. Synthesis and Application of Activity-Based Probes for Proteases
Tim Van Kersavond, Minh T. N. Nguyen, and Steven H. L. Verhelst