Plant Pattern Recognition Receptors: Methods and Protocols

Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors.- Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses.- Methods of Isolation and Characterization of Oligogalacturonide Elicitors.- Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation.- Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants.- Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors.- Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions.- In Vitro Ubiquitination Activity Assays in Plant Immune Responses.- Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily.- Identification of MAPK Substrates using Quantitative Phosphoproteomics.- Analysis of PAMP-Triggered ROS Burst in Plant Immunity.- LeEIX2 interactors' Analysis and EIX-mediated Response Measurement.- MAPK Assays in MAMP-PRR Signal Transduction Pathway in Arabidopsis.- CDPK Activation in PRR Signaling.- Protein Kinase Assays.- Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, during Bacterial Infection in Arabidopsis.- Quantitative Evaluation of Plant Actin Cytoskeletal Organization during Immune Signaling.- Network Reconstitution for Quantitative Subnetwork Interaction Analysis.- Stomatal Bioassay to Characterize Bacterial-stimulated PTI at the Pre-invasion Phase of Infection.- Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response.- Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato.- Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae.- Small RNA and mRNA Profiling of Arabidopsis Infected by Phytophthora.- Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants.- Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing.- Chitin-triggered MAPK activation and ROS generation in Rice Suspension-cultured Cells.- Chitin Induced Responses in the Moss Physcomitrella patens.- Methods to Quantify PAMP-Triggered MAP Kinase Phosphorylation, Oxidative Burst, and Lignification in Brassicas.- Effectoromics-Based Identification of Cell Surface Receptors in Potato.